Guo Jin, Ling Shengjie, Li Wenyi, Chen Ying, Li Chunmei, Omenetto Fiorenzo G, Kaplan David L
Department of Biomedical Engineering, Tufts University, MA 02155, USA.
Adv Funct Mater. 2018 May 9;28(19). doi: 10.1002/adfm.201800228. Epub 2018 Mar 25.
Well-designed micropatterns present in native tissues and organs involve changes in extracellular matrix compositions, cell types and mechanical properties to reflect complex biological functions. However, the design and fabrication of these micropatterns to meet task-specific biomedical applications remains a challenge. A design strategy to code and synthesize functional micropatterns is presented to engineer cell alignment through the integration of aqueous-peptide inkjet printing and site-specific biomineralization. The inkjet printing provides direct writing of macroscopic biosilica selective peptide-R5 patterns with micrometer-scale resolution on the surface of a biopolymer (silk) hydrogel. This is combined with biomineralization of the R5 peptide for site-specific growth of silica nanoparticles on the micropatterns, avoiding the use of harsh chemicals or complex processing. The functional micropatterned systems are used to align human mesenchymal stem cells and bovine serum albumin. This combination of peptide printing and site-specific biomineralization provides a new route for developing cost-effective micropatterns, with implications for broader materials designs. is demonstrated here. The functional micropatterned systems are used to align human mesenchymal stem cells and bovine serum albumin , avoiding the use of harsh chemicals or complex processing, while providing potential applications in developing cost-effective micropatterns to meet task-specific biomedical applications.
天然组织和器官中存在的精心设计的微图案涉及细胞外基质组成、细胞类型和机械性能的变化，以反映复杂的生物学功能。然而，设计和制造这些微图案以满足特定任务的生物医学应用仍然是一项挑战。本文提出了一种编码和合成功能性微图案的设计策略，通过整合水性肽喷墨打印和位点特异性生物矿化来设计细胞排列。喷墨打印可在生物聚合物（丝绸）水凝胶表面以微米级分辨率直接书写宏观生物二氧化硅选择性肽-R5图案。这与R5肽的生物矿化相结合，用于在微图案上进行二氧化硅纳米颗粒的位点特异性生长，避免使用苛刻的化学物质或复杂的加工过程。功能性微图案系统用于使人间充质干细胞和牛血清白蛋白排列。肽打印和位点特异性生物矿化的这种结合为开发具有成本效益的微图案提供了一条新途径，对更广泛的材料设计具有启示意义。本文对此进行了展示。功能性微图案系统用于使人间充质干细胞和牛血清白蛋白排列，避免使用苛刻的化学物质或复杂的加工过程，同时在开发具有成本效益的微图案以满足特定任务的生物医学应用方面具有潜在应用。
