Alikani Mina, Willadsen Steen M
Gamete and Embryo Research Laboratory, Institute for Reproductive Medicine and Science of Saint Barnabas, 101 Old Short Hills Road, Suite 501, West Orange, NJ 07052 USA.
Reprod Biomed Online. 2002 Jul-Aug;5(1):56-8. doi: 10.1016/s1472-6483(10)61599-4.
This study examined the developmental capacity of aggregates of surviving mono-nucleated cells isolated from several non-viable human embryos on day 3 or day 4 after fertilization. The results clearly demonstrate that some blastomeres from non-viable embryos do indeed maintain their developmental potential and regulatory capacity to the extent of being able to contribute to a normally organized blastocyst, with as many as 90% diploid cells. Although the chimaeric nature of such blastocysts excludes them from use in therapeutic IVF, they are of particular relevance to the discussion of embryonic and trophectodermal stem cell line production.
本研究检测了从受精后第3天或第4天的几个不可育人类胚胎中分离出的存活单核细胞聚集体的发育能力。结果清楚地表明，一些来自不可育胚胎的卵裂球确实保持了它们的发育潜能和调控能力，能够发育成一个正常组织的囊胚，其中二倍体细胞多达90%。尽管这种囊胚的嵌合性质使其不能用于治疗性体外受精，但它们与胚胎和滋养外胚层干细胞系产生的讨论特别相关。
