Koliakos George G, Konstas Anastasios G P, Schlötzer-Schrehardt Ursula, Bufidis Theodoros, Georgiadis Nikolaos, Ringvold Amund
Department of Biological Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Am J Ophthalmol. 2002 Dec;134(6):879-83. doi: 10.1016/s0002-9394(02)01797-x.
To investigate whether there is a role for ascorbic acid in the development of exfoliation syndrome (XFS).
A case-control study was undertaken that included consecutive patients with and without XFS in whom cataract surgery was indicated. Patients with ophthalmic conditions other than XFS and conditions that may influence ascorbic acid levels were excluded.
A prospective institutional study was undertaken. A small volume of aqueous humor was aspirated at the beginning of phacoemulsification cataract surgery. Eighty aqueous samples, 40 samples from 40 eyes of 40 cataract patients with XFS and 40 samples from 40 eyes of 40 age matched cataract patients without XFS, were collected and analyzed. Ascorbic acid concentration was evaluated in the aqueous samples with a microplate assay method.
The mean +/- SD concentration of ascorbic acid in the aqueous from patients with XFS (0.86 +/- 0.43 mM; range, 0.12 to 1.7 mM) was significantly lower than the concentration of ascorbic acid found in the aqueous of age-matched control patients (1.15 +/- 0.50 mM; range 0.42 to 3.1 mM; P =.0068). Total mean protein concentration was found to be significantly higher in the XFS group (481.1 +/- 196.8 pg/dl versus 336.3 +/- 86.4 pg/dl in the controls; P <.0001). Nevertheless, no correlation could be established between ascorbic level and protein concentration.
A significantly reduced mean level of ascorbic acid was observed in the aqueous humor of patients with XFS. In view of the fact that ascorbic acid is a major protective factor against free radical action, a role for free radical action is possible in the pathobiology of XFS.
研究抗坏血酸在剥脱综合征（XFS）发病过程中是否起作用。
进行了一项病例对照研究，纳入了有和没有XFS且需行白内障手术的连续患者。排除患有除XFS以外的眼科疾病以及可能影响抗坏血酸水平的疾病的患者。
进行了一项前瞻性机构研究。在白内障超声乳化手术开始时抽取少量房水。收集并分析了80份房水样本，其中40份来自40例患有XFS的白内障患者的40只眼睛，另外40份来自40例年龄匹配的无XFS的白内障患者的40只眼睛。采用微孔板分析法评估房水样本中的抗坏血酸浓度。
XFS患者房水中抗坏血酸的平均浓度±标准差为（0.86±0.43 mM；范围为0.12至1.7 mM），显著低于年龄匹配的对照患者房水中抗坏血酸的浓度（1.15±0.50 mM；范围为0.42至3.1 mM；P = 0.0068）。发现XFS组的总平均蛋白浓度显著高于对照组（481.1±196.8 pg/dl，而对照组为336.3±86.4 pg/dl；P < 0.0001）。然而，抗坏血酸水平与蛋白浓度之间未发现相关性。
在XFS患者的房水中观察到抗坏血酸的平均水平显著降低。鉴于抗坏血酸是对抗自由基作用的主要保护因子，自由基作用可能在XFS的病理生物学中起作用。
