Fiertak A, Kilarski W M
Department of Cytology and Histology, Institute of Zoology, Jagiellonian University, 30-060 Krakow, R. Ingardena 6, Poland.
Cell Mol Life Sci. 2002 Oct;59(10):1724-33. doi: 10.1007/pl00012500.
The aim of this work was to show differences in the terminal and subterminal sugar composition of carbohydrate chains of glycoconjugates produced by the goblet cells of the intestines of four cyprinids. We analysed intestines of two herbivorous species--sneep and grass carp--and two omnivorous ones--chub and common carp. We compared four intestinal regions of every studied species. In every region, the presence of neutral and acidic glycoconjugates was confirmed. The smallest amount of acidic glycoconjugates was present in the second region of sneep intestine. Sulphated glycoconjugates were absent in the third and fourth region of chub intestine. Lectin histochemistry provided evidence for the presence of beta-D-galactose, a-N-acetylgalactosamine, beta-N-acetylglucosamine and sialic acids. Additionally, the occurrence of alpha-L-fucose in the goblet cells of chub, grass carp and sneep was confirmed. We tried to correlate the pattern of glycoconjugate glycosylation with feeding habits of the studied fishes.
这项工作的目的是展示四种鲤科鱼类肠道杯状细胞产生的糖缀合物碳水化合物链末端和亚末端糖组成的差异。我们分析了两种草食性鱼类——斜齿鳊和草鱼,以及两种杂食性鱼类——圆腹雅罗鱼和鲤鱼的肠道。我们比较了每个研究物种的四个肠道区域。在每个区域,均证实存在中性和酸性糖缀合物。斜齿鳊肠道第二个区域的酸性糖缀合物含量最少。圆腹雅罗鱼肠道第三和第四区域不存在硫酸化糖缀合物。凝集素组织化学证明了β-D-半乳糖、α-N-乙酰半乳糖胺、β-N-乙酰葡糖胺和唾液酸的存在。此外,还证实了圆腹雅罗鱼、草鱼和斜齿鳊的杯状细胞中存在α-L-岩藻糖。我们试图将糖缀合物糖基化模式与所研究鱼类的摄食习性联系起来。