通过对ospC基因和rrf（5S）-rrl（23S）基因间隔区进行PCR-rFLP分析，对从韩国忠州的日本硬蜱和黑线姬鼠中分离出的阿氏疏螺旋体进行鉴定。

Characterization of Borrelia afzelii isolated from Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, by PCR-rFLP analyses of ospC gene and rrf (5S)-rrl (23S) intergenic spacer.

作者信息

Lee Seung-Hyun, Jung Kwang-Don, Lee Jung-Hee, Kim Sang-Choin, Kim Jong-Hyun, Jang Won-Jong, Park Kyung-Hee

机构信息

Department of Microbiology, College of Medicine, Konkuk University, Chungju, Chungchongbuk-Do 380-701, Korea.

出版信息

Microbiol Immunol. 2002;46(10):677-83. doi: 10.1111/j.1348-0421.2002.tb02751.x.

DOI:10.1111/j.1348-0421.2002.tb02751.x

PMID:12477246

Abstract

Nine Borrelia afzelii strains, which had been isolated from two vectors, Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, were characterized by PCR-RFLP analyses of ospC genes and rrf (5S)-rrl (23S) intergenic spacer. DraI restriction patterns of Chungju strains were identical to those of B. afzelii VS461. But MseI restriction patterns of rrf (5S)-rrl (23S) intergenic spacer genes of KK2, KM4, KK5 differed from those of previously reported B. burgdorferi sensu lato strains. Nine Chungju strains were classified with four distinct ospC RFLP patterns, which differed from the eight ospC RFLP patterns (A-1 to A-8) of previously reported B. afzelii. Moreover, five additional restriction patterns were deduced from published ospC sequences of reference strains. These results suggest that Chungju strains are very heterogeneous.

摘要

从韩国忠州的两种媒介物——日本硬蜱和黑线姬鼠中分离出的9株阿氏疏螺旋体菌株，通过对ospC基因和rrf(5S)-rrl(23S)基因间隔区进行PCR-RFLP分析来进行特征鉴定。忠州菌株的DraI限制性酶切图谱与阿氏疏螺旋体VS461的图谱相同。但是，KK2、KM4、KK5的rrf(5S)-rrl(23S)基因间隔区基因的MseI限制性酶切图谱与先前报道的广义伯氏疏螺旋体菌株不同。9株忠州菌株被分为4种不同的ospC RFLP图谱类型，这与先前报道的阿氏疏螺旋体的8种ospC RFLP图谱类型（A-1至A-8）不同。此外，从参考菌株已发表的ospC序列中推导得出另外5种限制性酶切图谱类型。这些结果表明忠州菌株具有高度的异质性。