通过rrf(5S)-rrl(23S)基因间隔区扩增子的限制性片段长度多态性对从日本狸蜱、乌鸫蜱和柱形蜱中分离出的疏螺旋体进行鉴定。
Characterization of Borrelia sp. isolated from Ixodes tanuki, I. turdus, and I. columnae in Japan by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons.
作者信息
Masuzawa T, Komikado T, Iwaki A, Suzuki H, Kaneda K, Yanagihara Y
机构信息
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
出版信息
FEMS Microbiol Lett. 1996 Aug 15;142(1):77-83. doi: 10.1111/j.1574-6968.1996.tb08411.x.
Borrelia isolated from various sources in Japan, including rare species of ixodid ticks, Ixodes tanuki, I. turdus, and I. columnae, were characterized by restriction fragment length polymorphism analysis and sequencing analysis of the 5S-23S rRNA intergenic spacer amplicon. Borrelia sp. isolated from I. tanuki, I. turdus and I. columnae generated restriction fragment length polymorphism patterns different from those of known B. burgdorferi sensu lato isolates previously reported. Furthermore, some B. afzelii and B. garinii isolated in Japan showed unique RFLP patterns which were not observed among European B. afzelii and B. garinii.
从日本的各种来源分离出的疏螺旋体，包括罕见的硬蜱物种，如貉硬蜱、欧夜鹰硬蜱和柱形硬蜱，通过对5S-23S rRNA基因间隔区扩增子进行限制性片段长度多态性分析和测序分析来进行特征鉴定。从貉硬蜱、欧夜鹰硬蜱和柱形硬蜱中分离出的疏螺旋体产生的限制性片段长度多态性模式与先前报道的已知狭义伯氏疏螺旋体分离株不同。此外，在日本分离出的一些阿氏疏螺旋体和伽氏疏螺旋体显示出独特的限制性片段长度多态性模式，这些模式在欧洲的阿氏疏螺旋体和伽氏疏螺旋体中未观察到。
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