Choi Yeon-Joo, Han Seung-Hoon, Park Jin-Mi, Lee Kyung-Min, Lee Eun-Mi, Lee Seung-Hyun, Song Hyeon-Je, Koh Young-Sang, Lee Keun-Wha, Jang Won-Jong, Park Kyung-Hee
Department of Microbiology, College of Medicine, Konkuk University, Seoul, Republic of Korea.
Microbiol Immunol. 2007;51(12):1201-7. doi: 10.1111/j.1348-0421.2007.tb04015.x.
Borrelia afzelii nucleic acids were detected in the sera of febrile disease patients by a nested PCR that targeted the rrf (5S)-rrl (23S) spacer of B. burgdorferi sensu lato. The B. afzelii-specific DNA fragment was detected in 8 out of 283 sera which were proven to have immunoglobulin G or M antibodies against B. burgdorferi antigens through IFA. The results were further confirmed through restriction fragment length polymorphism and sequencing analysis of the DNA fragments. The results indicated for the first time that Lyme borreliosis is prevalent in Korea.
通过针对伯氏疏螺旋体狭义种rrf（5S）-rrl（23S）间隔区的巢式PCR，在发热性疾病患者的血清中检测到阿氏疏螺旋体核酸。在283份血清中的8份中检测到阿氏疏螺旋体特异性DNA片段，这些血清经间接荧光抗体试验证实含有抗伯氏疏螺旋体抗原的免疫球蛋白G或M抗体。通过对DNA片段的限制性片段长度多态性分析和测序分析进一步证实了结果。结果首次表明莱姆病在韩国流行。
