Demidov Vadim V
Center for Advanced Biotechnology, Boston University, 36 Cummington Street, Boston, MA 02215, USA.
Trends Biotechnol. 2003 Jan;21(1):4-7. doi: 10.1016/s0167-7799(02)00008-2.
In some aspects, homogeneous (all-in-solution) nucleic acid hybridization assays are superior to the traditionally used heterogeneous (solution-to-surface) alternatives. Profluorescent probes, which reveal fluorescence enhancement or fluorescence polarization upon their binding to DNA and RNA targets, are a paradigm for the real-time sequence-specific homogeneous detection of nucleic acids. A variety of such DNA or RNA-derived probes of different constructs has already been developed with numerous applications. However, the recent additions to the field - locked nucleic acids (LNAs) and peptide nucleic acids (PNAs) - significantly increase the potential of profluorescent probes and provide a robust impulse for their new uses.
在某些方面，均相（全溶液）核酸杂交分析优于传统使用的异相（溶液到表面）方法。前荧光探针在与DNA和RNA靶标结合时会显示荧光增强或荧光偏振，是核酸实时序列特异性均相检测的范例。已经开发了多种不同构建体的此类DNA或RNA衍生探针，并具有众多应用。然而，该领域最近新出现的锁核酸（LNA）和肽核酸（PNA）显著提高了前荧光探针的潜力，并为其新用途提供了强大动力。
