Department of Chemical Engineering, California Institute for Quantitative Biosciences, and Howard Hughes Medical InstituteUniversity of California, Berkeley, California 94720, United States.
Nano Lett. 2010 Nov 10;10(11):4697-701. doi: 10.1021/nl102986v.
The ability to strongly and sequence-specifically attach modifications such as fluorophores and haptens to individual double-stranded (ds) DNA molecules is critical to a variety of single-molecule experiments. We propose using modified peptide nucleic acids (PNAs) for this purpose and implement them in two model single-molecule experiments where individual DNA molecules are manipulated via microfluidic flow and optical tweezers, respectively. We demonstrate that PNAs are versatile and robust sequence-specific tethers.
将诸如荧光团和半抗原等修饰物强烈且序列特异性地连接到各个双链 (ds) DNA 分子上的能力对于各种单分子实验至关重要。我们建议为此目的使用修饰的肽核酸 (PNA)，并在两个模型单分子实验中实施它们，其中分别通过微流控和光学镊子操纵单个 DNA 分子。我们证明了 PNA 是多功能且稳健的序列特异性连接物。
