Nakayama M, Haratake M, Ono M, Koiso T, Harada K, Nakayama H, Yahara S, Ohmomo Y, Arano Y
Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki, 852-8131, Japan.
Appl Radiat Isot. 2003 Jan;58(1):9-14. doi: 10.1016/s0969-8043(02)00268-3.
A macroporous styrene-divinylbenzene copolymer containing N-methylglucamine groups was selected for a new 68Ge/68Ga generator system. This resin packed into a column effectively adsorbed the parent nuclide 68Ge. The daughter 68Ga was eluted from the resin with a solution of a low-affinity gallium chelating ligand such as citric or phosphoric acid. The 68Ge leakage was less than 0.0004% of the 68Ge adsorbed on the resin. By simple mixing of transferrin and desferoxamine conjugated HSA and IgG with the eluate from the column, 68Ga-labeling was completed in high yield.
一种含有N-甲基葡糖胺基团的大孔苯乙烯-二乙烯基苯共聚物被选用于一种新型的68Ge/68Ga发生器系统。这种填充在柱中的树脂有效地吸附了母体核素68Ge。子体68Ga用低亲和力的镓螯合配体溶液（如柠檬酸或磷酸）从树脂上洗脱下来。68Ge的泄漏量小于吸附在树脂上的68Ge的0.0004%。通过将转铁蛋白和去铁胺共轭的人血清白蛋白及免疫球蛋白与柱洗脱液简单混合，以高产率完成了68Ga标记。
