Bramley T A
Mol Cell Endocrinol. 1976 Feb;4(3):151-5. doi: 10.1016/0303-7207(76)90034-4.
Five-day-old female mice were injected subcutaneously with 100 mug of testosterone benzoate in oil, or with oil only. At various ages thereafter, they received either 5 I.U. human chorionic gonadotropin (hCG) per 10 g body weight or saline only, and were killed 24 h later. Alkaline phosphatase activity was measured in whole ovarian homogenates. Neonatal androgenization failed to affect the early phases of ovarian responsiveness, but selectively abolished both the normal rise in alkaline phosphatase which precedes the onset of puberty and the responsiveness of the enzyme to hCG stimulation at this time.
给5日龄雌性小鼠皮下注射100微克溶于油中的苯丙酸睾酮，或仅注射油。此后在不同年龄，它们每10克体重接受5国际单位人绒毛膜促性腺激素（hCG）或仅接受生理盐水，并在24小时后处死。测定整个卵巢匀浆中的碱性磷酸酶活性。新生期雄激素化未能影响卵巢反应性的早期阶段，但选择性地消除了青春期开始前碱性磷酸酶的正常升高以及此时该酶对hCG刺激的反应性。
