Morzycka Bozena
Plant Protection Institute in Poznan, Experimental Field Station in Bialystok, ul. Chelmonskiego 22, 15-195 Bialystok, Poland.
J Chromatogr A. 2002 Dec 27;982(2):267-73. doi: 10.1016/s0021-9673(02)01505-4.
A simple multiresidue method for the determination of insecticides in honeybees is described. The developed method is based on the matrix solid-phase dispersion technique. A total number of 12 insecticides (azinfos-methyl, buprofezin, chlorpyriphos, chlorpyriphos-methyl, diazinon, ethion, fenitrothion, fipronil, methidathion, phosalone, pirimicarb, propoxur) used on flowering fields are determined by this method. The method uses Florisil and silica as dispersing agents, alumina and silica as cleanup adsorbents and a low polarity solvent system to elute pesticide residues from the honeybee samples. The insecticides were quantified using capillary gas chromatography with a nitrogen-phosphorus detector. The method has shown good recovery (70-110%) for various levels of spiked samples (0.01-1.0 mg/kg). The relative standard deviations were in the range of 2-8% for all pesticides studied. The limits of detection were in the range of 0.005-0.05 mg/kg. The procedure can be applied for the determination of residues of low-polarity and medium polarity pesticides in honeybee samples.
本文描述了一种用于测定蜜蜂体内杀虫剂的简单多残留方法。所开发的方法基于基质固相分散技术。该方法可测定在开花田使用的总共12种杀虫剂（甲基谷硫磷、噻嗪酮、毒死蜱、甲基毒死蜱、二嗪农、乙硫磷、杀螟硫磷、氟虫腈、杀扑磷、伏杀硫磷、抗蚜威、残杀威）。该方法使用弗罗里硅土和硅胶作为分散剂，氧化铝和硅胶作为净化吸附剂，并采用低极性溶剂系统从蜜蜂样品中洗脱农药残留。使用配有氮磷检测器的毛细管气相色谱法对杀虫剂进行定量。该方法对不同加标水平（0.01 - 1.0 mg/kg）的样品显示出良好的回收率（70 - 110%）。所研究的所有农药的相对标准偏差在2 - 8%范围内。检测限在0.005 - 0.05 mg/kg范围内。该方法可用于测定蜜蜂样品中低极性和中等极性农药的残留。
