Fan J, Minchella D J, Day S R, McManus D P, Tiu W U, Brindley P J
Molecular Parasitology Unit and Australian Centre for International & Tropical Health & Nutrition, The Queensland Institute of Medical Research, Brisbane, Queensland, Australia.
Biochem Biophys Res Commun. 1998 Nov 18;252(2):348-56. doi: 10.1006/bbrc.1998.9491.
Here we report 658 expressed sequence tags (ESTs) generated from the 5'-termini of clones randomly selected from directional cDNA libraries constructed from mRNAs from three developmental stages of Schistosoma japonicum. Putative identifications were assigned to 46. 2% of the ESTs; 6.4% were previously known from S. japonicum, 5.6% were previously known from S. mansoni, 34.2% were known from other organisms, and the remaining 53.8% may represent S. japonicum-specific genes. These 658 ESTs appeared to be derived from 457 unique genes, which together represent 2 to 3% of the 15,000 to 20,000 genes predicted to occur in the schistosome genome.
我们在此报告了658个表达序列标签（EST），这些标签来自从日本血吸虫三个发育阶段的mRNA构建的定向cDNA文库中随机选择的克隆的5'末端。46.2%的EST被赋予了推定的鉴定结果；6.4%先前已知来自日本血吸虫，5.6%先前已知来自曼氏血吸虫，34.2%已知来自其他生物，其余53.8%可能代表日本血吸虫特异性基因。这658个EST似乎来自457个独特基因，它们共同代表了血吸虫基因组中预计出现的15000至20000个基因中的2%至3%。
