Hargrave Kimberly M, Li ChangLong, Meyer Brett J, Kachman Stephen D, Hartzell Diane L, Della-Fera Mary Anne, Miner Jess L, Baile Clifton A
Department of Animal Science, University of Nebraska, Lincoln, USA.
Obes Res. 2002 Dec;10(12):1284-90. doi: 10.1038/oby.2002.174.
To compare the effectiveness of a conjugated linoleic acid (CLA) isomer mixture (mCLA) with each main isomer [trans-10,cis-12 CLA (CLA10,12) and cis-9,trans-11 CLA (CLA9,11)] in causing body lipid loss and adipose tissue apoptosis.
Mice selected over 16 generations for high (MH) or low (ML) energy expenditure and a control group (MC) were fed diets containing either soy oil or soy oil plus mCLA, CLA10,12, or CLA9,11 for 5 days in one study and 14 days in a second study.
Mice fed mCLA or CLA10,12 had less body lipid (p < 0.05), smaller retroperitoneal fat pads (p < 0.05), and ate less (p < 0.01) than mice fed no CLA or CLA9,11 for 5 days. Mice consuming 1% mCLA or 0.5% CLA10,12 gained less weight (p < 0.01) and had less body lipid (p < 0.05) and smaller epididymal (p < 0.05) and retroperitoneal fat pads (p < 0.01) than mice consuming either control or 0.5% CLA9,11-containing diets for 14 days. Only mCLA and CLA10,12 increased apoptosis in retroperitoneal fat pads (p < 0.01). The effects of mCLA and CLA10,12 were independent of genetic line except for the effect on adipocyte apoptosis. Mice of the MH line were slightly less sensitive than MC or ML mice to CLA-induced adipose tissue apoptosis.
CLA10,12, but not CLA9,11, can induce both body fat loss and adipose apoptosis. Although mice of a genotype with less body fat and greater metabolic rate and feed intake appear less sensitive, these CLA effects are robust for mice of varying metabolic background.
比较共轭亚油酸（CLA）异构体混合物（mCLA）与各主要异构体[反式-10，顺式-12 CLA（CLA10,12）和顺式-9，反式-11 CLA（CLA9,11）]在引起机体脂质损失和脂肪组织凋亡方面的效果。
在一项研究中，将经过16代选育的高能量消耗（MH）或低能量消耗（ML）小鼠以及一个对照组（MC）小鼠，喂食含大豆油或含大豆油加mCLA、CLA10,12或CLA9,11的饮食5天；在第二项研究中喂食14天。
在5天的实验中，喂食mCLA或CLA10,12的小鼠比未喂食CLA或CLA9,11的小鼠体脂更少（p<0.05），腹膜后脂肪垫更小（p<0.05），进食更少（p<0.01）。在14天的实验中，食用1% mCLA或0.5% CLA10,12的小鼠比食用对照饮食或含0.5% CLA9,11饮食的小鼠体重增加更少（p<0.01），体脂更少（p<0.05），附睾脂肪垫（p<0.05）和腹膜后脂肪垫更小（p<0.01）。只有mCLA和CLA10,12增加了腹膜后脂肪垫中的细胞凋亡（p<0.01）。除了对脂肪细胞凋亡的影响外，mCLA和CLA10,12的作用与遗传品系无关。MH品系的小鼠对CLA诱导的脂肪组织凋亡的敏感性略低于MC或ML小鼠。
CLA10,12而非CLA9,11可诱导体脂减少和脂肪细胞凋亡。尽管具有较低体脂、较高代谢率和采食量的基因型小鼠似乎敏感性较低，但这些CLA对不同代谢背景的小鼠的作用是显著的。
