Kopwillem A, Merriman W G, Cuddeback R M, Smolka A J, Bier M
J Chromatogr. 1976 Mar 3;118(1):34-46. doi: 10.1016/s0021-9673(00)81028-6.
Analytical and preparative isotachophoresis has been carried out using amino acids and peptides as discrete spacers in contrast to the usually employed continuous mobility spectrum Ampholine. Analytical isotachoresis in free solution, using the LKB Tachophor, demonstrated the separation of human serum into distinct mobility subgroups, n spacers giving rise to n + 1 protein subgroups. Preparative fractionation on polyacrylamide gel was carried out on the LKB Uniphor using threonine and glycine as spacers. Immunoelectrophoretic analysis showed that eight out of ten proteins assayed were clearly resolved in the three subgroups obtained, thus demonstrating the sharpness of isotachophoretic resolution.
与通常使用的具有连续迁移谱的两性电解质不同，已经使用氨基酸和肽作为离散间隔物进行了分析型和制备型等速电泳。使用LKB Tachophor在自由溶液中进行分析型等速电泳，结果表明人血清可分离成不同的迁移亚组，n个间隔物会产生n + 1个蛋白质亚组。使用苏氨酸和甘氨酸作为间隔物，在LKB Uniphor上进行聚丙烯酰胺凝胶的制备分级分离。免疫电泳分析表明，在所检测的十种蛋白质中，有八种在获得的三个亚组中得到了清晰的分离，从而证明了等速电泳分辨率的清晰度。
