Musalmah Mazlan, Fairuz Abd Halim, Gapor Mat Top, Ngah Wan Zurinah Wan
Department of Biochemistry, Medical Faculty, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Asia Pac J Clin Nutr. 2002;11 Suppl 7:S448-51. doi: 10.1046/j.1440-6047.11.s.7.6.x.
Vitamin E is composed of various subfamilies that include tocopherols and tocotrienols. These compounds have antioxidant properties but differ in structure, dietary source and potency. In this study we evaluated the efficacy of alpha-tocopherol as an antioxidant and its role in wound closure in normal and streptozotocin-induced diabetic rats. The healing of 6 cm linear incisions created on the back of each male Sprague-Dawley rat (250-300 g) was monitored by measuring the length of the wounds daily. The rats were divided into two categories; normal and streptozotocin-induced diabetic rats. For each category, the animals were further divided into two groups; those untreated and those receiving 200 mg/kg bodyweight alpha-tocopherols daily by oral gavage. All rats were fed standard food and water ad libitum. Blood samples were taken at 0, 5 and 10 days after the wounds were created for the determination of malondialdehyde levels and red cell superoxide dismutase, catalase and glutathione peroxidase activities. The results showed that alpha-tocopherol reduced plasma malondialdehyde levels, increased glutathione peroxidase activity and accelerated the rate of wound closure in treated rats.
维生素E由多种亚家族组成，包括生育酚和生育三烯酚。这些化合物具有抗氧化特性，但在结构、饮食来源和效力方面存在差异。在本研究中，我们评估了α-生育酚作为抗氧化剂的功效及其在正常和链脲佐菌素诱导的糖尿病大鼠伤口愈合中的作用。通过每天测量伤口长度来监测在每只雄性Sprague-Dawley大鼠（250-300克）背部创建的6厘米线性切口的愈合情况。大鼠分为两类：正常大鼠和链脲佐菌素诱导的糖尿病大鼠。对于每一类，动物进一步分为两组：未治疗组和每天通过口服灌胃接受200毫克/千克体重α-生育酚的组。所有大鼠自由采食标准食物和饮水。在伤口形成后0、5和10天采集血样，用于测定丙二醛水平以及红细胞超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性。结果表明，α-生育酚降低了血浆丙二醛水平，增加了谷胱甘肽过氧化物酶活性，并加速了治疗组大鼠的伤口闭合速度。
