体内筛选具有高水平氟喹诺酮耐药性且与gyrA突变及CmeABC外排泵功能相关的弯曲杆菌分离株。
In vivo selection of Campylobacter isolates with high levels of fluoroquinolone resistance associated with gyrA mutations and the function of the CmeABC efflux pump.
作者信息
Luo Naidan, Sahin Orhan, Lin Jun, Michel Linda O, Zhang Qijing
机构信息
Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, Ohio 44691, USA.
出版信息
Antimicrob Agents Chemother. 2003 Jan;47(1):390-4. doi: 10.1128/AAC.47.1.390-394.2003.
Abstract
Enrofloxacin treatment of chickens infected with fluoroquinolone(FQ)-sensitive Campylobacter promoted the emergence of FQ-resistant Campylobacter mutants which propagated in the intestinal tract and recolonized the chickens. The recovered isolates were highly resistant to quinolone antibiotics but remained susceptible to non-FQ antimicrobial agents. Specific single-point mutations in the gyrA gene and the function of the CmeABC efflux pump were linked to the acquired FQ resistance. These results reveal that Campylobacter is hypermutable in vivo under the selection pressure of FQ and highlight the need for the prudent use of FQ antibiotics.
摘要
恩诺沙星治疗感染对氟喹诺酮（FQ）敏感的弯曲杆菌的鸡，促使对FQ耐药的弯曲杆菌突变体出现，这些突变体在肠道中繁殖并重新定殖于鸡体内。回收的分离株对喹诺酮类抗生素高度耐药，但对非FQ抗菌剂仍敏感。gyrA基因中的特定单点突变和CmeABC外排泵的功能与获得性FQ耐药性有关。这些结果表明，弯曲杆菌在FQ的选择压力下在体内具有高度变异性，并突出了谨慎使用FQ抗生素的必要性。
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