Till Marisa, Robson Alice, Byrne Matthew J, Nair Asha V, Kolek Stefan A, Shaw Stewart Patrick D, Race Paul R
School of Biochemistry, University of Bristol.
J Vis Exp. 2013 Aug 31(78):50548. doi: 10.3791/50548.
Random microseed matrix screening (rMMS) is a protein crystallization technique in which seed crystals are added to random screens. By increasing the likelihood that crystals will grow in the metastable zone of a protein's phase diagram, extra crystallization leads are often obtained, the quality of crystals produced may be increased, and a good supply of crystals for data collection and soaking experiments is provided. Here we describe a general method for rMMS that may be applied to either sitting drop or hanging drop vapor diffusion experiments, established either by hand or using liquid handling robotics, in 96-well or 24-well tray format.
随机微晶种矩阵筛选（rMMS）是一种蛋白质结晶技术，即在随机筛选中加入晶种。通过增加晶体在蛋白质相图亚稳区生长的可能性，常常能获得额外的结晶线索，提高所产生晶体的质量，并为数据收集和浸泡实验提供充足的晶体。在此，我们描述了一种适用于坐滴或悬滴气相扩散实验的rMMS通用方法，该实验可通过手工或使用液体处理机器人以96孔或24孔板的形式进行设置。
