通过随机微种子矩阵筛选提高蛋白质结晶成功率。
Improving the success rate of protein crystallization by random microseed matrix screening.
作者信息
Till Marisa, Robson Alice, Byrne Matthew J, Nair Asha V, Kolek Stefan A, Shaw Stewart Patrick D, Race Paul R
机构信息
School of Biochemistry, University of Bristol.
出版信息
J Vis Exp. 2013 Aug 31(78):50548. doi: 10.3791/50548.
Abstract
Random microseed matrix screening (rMMS) is a protein crystallization technique in which seed crystals are added to random screens. By increasing the likelihood that crystals will grow in the metastable zone of a protein's phase diagram, extra crystallization leads are often obtained, the quality of crystals produced may be increased, and a good supply of crystals for data collection and soaking experiments is provided. Here we describe a general method for rMMS that may be applied to either sitting drop or hanging drop vapor diffusion experiments, established either by hand or using liquid handling robotics, in 96-well or 24-well tray format.
摘要
随机微晶种矩阵筛选（rMMS）是一种蛋白质结晶技术，即在随机筛选中加入晶种。通过增加晶体在蛋白质相图亚稳区生长的可能性，常常能获得额外的结晶线索，提高所产生晶体的质量，并为数据收集和浸泡实验提供充足的晶体。在此，我们描述了一种适用于坐滴或悬滴气相扩散实验的rMMS通用方法，该实验可通过手工或使用液体处理机器人以96孔或24孔板的形式进行设置。
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