Zhou Jun-Fu, Chen Wei-Wei, Tong Gui-Zhong
The Second Affiliated Hospital, College of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, Zhejiang, China.
Biomed Environ Sci. 2003 Jun;16(2):95-104.
To estimate the impact of copying on the indoor air quality, and to investigate whether ozone emitted during such a process induces pathological oxidative stress and potential oxidative damage in the bodies of operators.
67 copying operators (CO) and 67 healthy volunteers (HV) were enrolled in a random control study, in which levels of lipoperoxide (LPO) in plasma and erythrocytes, and levels of vitamin C (VC), vitamin E (VE) and beta-carotene (beta-CAR) in plasma as well as activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in erythrocytes were determined by spectrophotometric methods.
Compared with the HV group, the average values of LPO in plasma and erythrocytes in the CO group were significantly increased (P<0.0001), while those of VC, VE and beta-CAR in plasma as well as those of SOD, CAT and GPX in erythrocytes in the CO group were significantly decreased (P<0.0001). Pearson product-moment correlation analysis showed that with increase of ozone level in copying sites and duration of exposure to ozone, the values of LPO in plasma and erythrocytes in the bodies of operators were gradually increased,while those of VC, VE, beta-CAR, SOD, CAT and GPX were decreased in the same manner. Odds ratio (OR) of risk of biochemical parameters reflecting potential oxidative damage of the copying operators ranged from 4.440 to 13.516, and 95% CI of OR was from 2.113 to 34.061. Reliability coefficient (alpha) of the biochemical parameters used to reflect the potential oxidative damage of the operators was 0.8156, standardized item alpha=0.9929, P<0.0001.
Findings in the present study suggest that there exist a series of free radical chain reactions and pathological oxidative stress induced by high dose ozone in the operators, thereby causing potential oxidative and lipoperoxidative damages in their bodies.
评估复印对室内空气质量的影响，并调查在此过程中释放的臭氧是否会在操作人员体内引发病理性氧化应激和潜在的氧化损伤。
67名复印操作人员（CO）和67名健康志愿者（HV）参与了一项随机对照研究，采用分光光度法测定血浆和红细胞中的过氧化脂质（LPO）水平、血浆中的维生素C（VC）、维生素E（VE）和β-胡萝卜素（β-CAR）水平以及红细胞中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）活性。
与HV组相比，CO组血浆和红细胞中LPO的平均值显著升高（P<0.0001），而CO组血浆中VC、VE和β-CAR以及红细胞中SOD、CAT和GPX的平均值显著降低（P<0.0001）。Pearson积矩相关分析表明，随着复印场所臭氧水平的升高和操作人员接触臭氧时间的延长，操作人员体内血浆和红细胞中LPO的值逐渐升高，而VC、VE、β-CAR、SOD、CAT和GPX的值则以相同方式降低。反映复印操作人员潜在氧化损伤的生化参数风险的比值比（OR）范围为4.440至13.516，OR的95%置信区间为2.113至34.061。用于反映操作人员潜在氧化损伤的生化参数的信度系数（α）为α=0.8156，标准化项目α=0.9929，P<0.0001。
本研究结果表明，高剂量臭氧在操作人员体内引发了一系列自由基链式反应和病理性氧化应激，从而在其体内造成潜在的氧化和过氧化损伤。
