Zhu You-Gen, Zhou Jun-Fu, Shan Wei-Ying, Zhou Pei-Su, Tong Gui-Zhong
Jinhua Municipal People's Hospital, 228 Xinhua Street, Jinhua 321000, Zhejiang, China.
Biomed Environ Sci. 2004 Dec;17(4):381-9.
To investigate whether photochemical smog emitted during the process of electric arc welding might cause oxidative stress and potential oxidative damage in the bodies of welding operators.
Seventy electric arc welding operators (WOs) and 70 healthy volunteers (HVs) were enrolled in a randomized controlled study design, in which the levels of vitamin C (VC) and vitamin E (VE) in plasma as well as the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and the level of lipoperoxide (LPO) in erythrocytes were determined by spectrophotometry.
Compared with the average values of the above experimental parameters in the HVs group, the average values of VC and VE in plasma as well as those of SOD, CAT and GPX in erythrocytes in the WOs group were significantly decreased (P < 0.005-0.0001), while the average value of LPO in erythrocytes in the WOs group was significantly increased (P < 0.0001). The findings from the partial correlation analysis on the controlling of age suggested that with a prolonged duration of exposure to photochemical smog the values of VC, VE, SOD, and GPX, except for CAT, in the WOs were decreased gradually (P < 0.05-0.005), the value of LPO in the WOs was increased gradually (P < 0.001), and that with the ozone dose increased in the air in each worksite VC, VE, SOD, CAT and GPX decreased (P < 0.005-0.001), but LPO increased (P < 0.001). The findings from the reliability analysis for the VC, VE, SOD, CAT, GPX, and LPO values which were used to reflect oxidative stress and potential oxidative damage in the WOs showed that the reliability coefficients' alpha (6 items) was 0.8021, P < 0.0001, and that the standardized item alpha was 0.9577, P < 0.0001.
Findings in the present study suggest that there exists an oxidative stress induced by long-term exposure to photochemical smog in the bodies of WOs, thereby causing potential oxidative and lipoperoxidative damages in their bodies.
探讨电弧焊过程中排放的光化学烟雾是否会对焊接操作人员身体造成氧化应激及潜在的氧化损伤。
采用随机对照研究设计，纳入70名电弧焊操作人员（WOs）和70名健康志愿者（HVs），通过分光光度法测定血浆中维生素C（VC）和维生素E（VE）水平以及红细胞中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）的活性，以及红细胞中脂质过氧化物（LPO）水平。
与HVs组上述实验参数的平均值相比，WOs组血浆中VC和VE的平均值以及红细胞中SOD、CAT和GPX的平均值显著降低（P<0.005 - 0.0001），而WOs组红细胞中LPO的平均值显著升高（P<0.0001）。控制年龄后的偏相关分析结果显示，随着接触光化学烟雾时间的延长，WOs组中除CAT外的VC、VE、SOD和GPX值逐渐降低（P<0.05 - 0.005），LPO值逐渐升高（P<0.001）；各工作场所空气中臭氧剂量增加时，VC、VE、SOD、CAT和GPX降低（P<0.005 - 0.001），但LPO升高（P<0.001）。用于反映WOs氧化应激和潜在氧化损伤的VC、VE、SOD、CAT、GPX和LPO值的信度分析结果显示，信度系数α（6项）为0.8021，P<0.0001，标准化项目α为0.9577，P<0.0001。
本研究结果表明，WOs体内存在长期接触光化学烟雾诱导的氧化应激，从而对其身体造成潜在的氧化和脂质过氧化损伤。
