Hulan H W, Kramer J K, Mahadevan S, Sauer F D
Lipids. 1976 Jan;11(1):9-15. doi: 10.1007/BF02532578.
The back and belly fat of pigs fed a diet containing 20% by wt rapeseed oil (22% erucic acid) for 16 weeks was rendered into oil. This rendered pig fat, which contained 5.6% erucic acid, was fed to male rats in three separate experiments at 20% by wt of the diet for 16 weeks. In experiment I rendered pig fat was compared only to Brassica campestris var. Span rapeseed oil containing 4.8% erucic acid. In experiments II and III, rendered pig fat was compared to commercial lard containing 0.2% docosenoic acid, commercial lard to which 5.4% free erucic acid was added, and Span rapeseed oil. There was no significant (P less than 0.01) differences observed in the level of erucic acid in the hearts of rats fed diets of rendered pig fat, Span rapeseed oil, or commercial lard plus erucic acid. However, the incidence (P less than 0.001) and severity (P less than 0.01) of cardiac lesions were significantly higher in Span rapeseed oil fed rats compared to rats fed control diets. The number of rats affected or the severity of lesions in the rendered pig fat fed group was not significantly different from controls. The results of this study indicate that the myocardial lesions associated with feeding 20% rapeseed oil diets are not related to the content of erucic acid per se. The possible reasons why rapeseed oil causes cardiac lesions in rats are discussed. It is suggested that a triglyceride imbalance in the oil might play an important role in causing these lesions in rats.
给猪喂食含20%（重量）菜籽油（芥酸含量22%）的日粮16周后，将其背部和腹部脂肪炼制成油。这种炼制成的猪脂肪含5.6%芥酸，在三项独立实验中，按日粮重量的20%喂给雄性大鼠，持续16周。在实验I中，仅将炼制成的猪脂肪与含4.8%芥酸的欧洲油菜籽油进行比较。在实验II和III中，将炼制成的猪脂肪与含0.2%二十二碳烯酸的市售猪油、添加了5.4%游离芥酸的市售猪油以及欧洲油菜籽油进行比较。在喂食炼制成的猪脂肪、欧洲油菜籽油或添加了芥酸的市售猪油日粮的大鼠心脏中，未观察到芥酸水平有显著差异（P小于0.01）。然而，与喂食对照日粮的大鼠相比，喂食欧洲油菜籽油的大鼠心脏病变的发生率（P小于0.001）和严重程度（P小于0.01）显著更高。喂食炼制成的猪脂肪组中受影响的大鼠数量或病变严重程度与对照组无显著差异。本研究结果表明，与喂食20%菜籽油日粮相关的心肌病变与芥酸本身的含量无关。讨论了菜籽油导致大鼠心脏病变的可能原因。有人提出，油中的甘油三酯失衡可能在导致大鼠这些病变中起重要作用。
