Metabolism of nitrosamines in vivo IV. Isolation of 3-hydroxy-1-nitrosopyrrolidine from rat urine after application of 1-nitrosopyrrolidine.

After i. p. application of 6 mg/30 muCi/kg 2,5- and 3,4--14C-nitrosopyrrolidine about 20% of the radioactivity is exhaled as 14-CO2 and about 7% is found in the urine. One of the urinary metabolites was identified by thin layer chromatography, combined GC/MS spectrometry and ultraviolet absorption as 3-hydroxy-1-nitrosopyrrolidine.