Moskaleva E Iu
Biull Eksp Biol Med. 1989 Sep;108(9):335-7.
The DNA structure of human peripheral blood lymphocytes was studied following activation by concanavalin A in vitro. DNA strand breaks were measured by the fluorometric method after DNA alkaline denaturation in cell lysates. Stimulation of lymphocytes proliferation was controlled by 3H-thymidine and 3H-uridine incorporation. It was shown that 1-3 hours after the addition of mitogens the quantity of alkali-labile sites in DNA increased. Cultivation of lymphocytes in vitro without mitogens was accompanied by gradual accumulation of alkali-labile sites in DNA and in 18-72 hours the DNA structure of lymphocytes in the culture was the same. It is possible that the rapid disturbances in the DNA secondary structure of lymphocytes following activation by mitogens is determined by the transmembrane signal transduction and is the one of the early events in genome activation.
研究了体外伴刀豆球蛋白A激活后人外周血淋巴细胞的DNA结构。在细胞裂解物中进行DNA碱性变性后，通过荧光法测量DNA链断裂。通过3H-胸腺嘧啶核苷和3H-尿苷掺入来控制淋巴细胞增殖的刺激。结果表明，添加促有丝分裂原后1 - 3小时，DNA中碱不稳定位点的数量增加。在无促有丝分裂原的情况下体外培养淋巴细胞，DNA中碱不稳定位点会逐渐积累，培养18 - 72小时后，培养物中淋巴细胞的DNA结构相同。有可能促有丝分裂原激活后淋巴细胞DNA二级结构的快速紊乱是由跨膜信号转导决定的，并且是基因组激活的早期事件之一。
