链霉菌的分离与鉴定及其胞外水溶性蓝色色素的测定

Isolation and identification of Streptomyces sp. and assay of its exocellular water-soluble blue pigments.

作者信息

Lu Ling, Cui H L, Chen Y N, Yuan S

机构信息

College of Life Sciences, Nanjing Normal University, Nanjing, 210 097, People's Republic of China.

出版信息

Folia Microbiol (Praha). 2002;47(5):493-8. doi: 10.1007/BF02818787.

DOI:10.1007/BF02818787

PMID:12503393

Abstract

A bacterial strain producing a great amount of blue pigment during submerse fermentation was isolated and identified. Based on morphological characteristics, cell-wall chemotype and sequence of 16S rRNA gene, the strain should belong to the genus Streptomyces; it had 99.4% homology of 16S rRNA gene sequence with that of Streptomyces indigocolor. The pigment production by the strain was affected by carbon and nitrogen sources. The main components of the pigment mixture (detected by HPLC and TLC) were tentatively classified as actinorhodin-related compounds. The pigment was relatively stable against light and higher temperature but was sensitive to low pH. The preliminary acute-toxicity determination showed that the pigment was nontoxic (LD50 > 15 mg/g).

摘要

分离并鉴定了一株在深层发酵过程中产生大量蓝色色素的细菌菌株。基于形态特征、细胞壁化学类型和16S rRNA基因序列，该菌株应属于链霉菌属；其16S rRNA基因序列与产靛青链霉菌的序列具有99.4%的同源性。该菌株的色素产生受碳源和氮源的影响。色素混合物的主要成分（通过高效液相色谱法和薄层色谱法检测）初步归类为与放线紫红素相关的化合物。该色素对光和较高温度相对稳定，但对低pH敏感。初步急性毒性测定表明该色素无毒（半数致死量>15毫克/克）。