Department of Microbiology, Gulbarga University, Gulbarga, 585106 Karnataka, India.
Saudi J Biol Sci. 2013 Apr;20(2):163-8. doi: 10.1016/j.sjbs.2013.01.004. Epub 2013 Feb 1.
Colors from natural sources are gaining popularity because synthetic colors are carcinogenic. Natural colors are obtained from plants or microorganisms. Pigments produced by microorganisms have advantages over plant pigments, due to their ease of use and reliability. In the present study, a blue pigment producing actinomycete klmp33 was isolated from the Gulbarga region in India. The isolate was identified based on morphologic, microscopic, and biochemical characterization, and 16S rRNA sequencing. Phylogenetic analysis of the isolates showed a close relationship with Streptomyces coelicolor. Pigment produced by the isolate was analyzed using UV-visible spectroscopy, thin-layer chromatography, Fourier transform infrared and liquid chromatography/mass spectroscopy analysis, and was identified as γ actinorhodin. γ-Actinorhodin is used as a pH indicator which deviates from acid to non-acid. Moreover, it subrogates synthetic dye.
天然来源的颜色越来越受欢迎,因为合成颜色具有致癌性。天然颜色是从植物或微生物中获得的。与植物色素相比,微生物产生的色素具有易用性和可靠性等优势。在本研究中,从印度古尔伯加地区分离到一株产生蓝色素的放线菌 klmp33。该分离株基于形态学、显微镜和生化特性以及 16S rRNA 测序进行鉴定。分离株的系统发育分析显示与变铅青链霉菌密切相关。使用紫外可见光谱、薄层层析、傅里叶变换红外和液相色谱/质谱分析对分离株产生的色素进行了分析,鉴定为γ-actinorhodin。γ-actinorhodin 可用作 pH 指示剂,从酸性变为非酸性。此外,它还替代了合成染料。