Chauhan Ved, Sheikh Ashfaq, Chauhan Abha, Tsiouris John, Malik Mazhar, Vaughan Michael
NYS Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA.
Biochimie. 2002 Oct;84(10):1031-4. doi: 10.1016/s0300-9084(02)00006-8.
During hibernation, fat is known to be the preferred source of energy. A detailed analysis of different phospholipids, as well as free and esterified cholesterol, was conducted to investigate lipid abnormalities during hibernation. The levels of total phospholipids and total cholesterol in the serum of black bears were found to increase significantly in hibernation as compared with the active state. Both free and esterified cholesterol were increased in the hibernating state in comparison with the active state (P < 0.05). The percentage increase during hibernation was more in free cholesterol (57%) than in esterified cholesterol (27%). Analysis of subclasses of serum phospholipids showed that choline containing phospholipids, i.e., sphingomyelin (SPG) (14%) and phosphatidylcholine (PC) (76%), are the major phospholipids in the serum of bear. The minor phospholipids included 8% of phosphatidylserine (PS) + phosphatidylinositol (PI), while phosphatidylethanolamine (PE) was only 2% of the total phospholipids. A comparison of phospholipid subclasses showed that PC, PS + PI and SPG were significantly increased, while PE was significantly decreased (P < 0.05) in the hibernating state as compared with the active state in black bears. These results suggest that the catabolism of phospholipids and cholesterol is decreased during hibernation in black bears, leading to their increased levels in the hibernating state as compared with the active state. In summary, our results indicate that serum cholesterol and phospholipid fractions (except PE) are increased during hibernation in bears. It is proposed that the increase of these lipids may be due to the altered metabolism of lipoproteins that are responsible for the clearance of the lipids.
在冬眠期间，脂肪被认为是首选的能量来源。为了研究冬眠期间的脂质异常情况，对不同的磷脂以及游离胆固醇和酯化胆固醇进行了详细分析。结果发现，与活跃状态相比，黑熊血清中的总磷脂和总胆固醇水平在冬眠期间显著升高。与活跃状态相比，冬眠状态下的游离胆固醇和酯化胆固醇均有所增加（P < 0.05）。冬眠期间游离胆固醇的增加百分比（57%）高于酯化胆固醇（27%）。血清磷脂亚类分析表明，含胆碱的磷脂，即鞘磷脂（SPG）（14%）和磷脂酰胆碱（PC）（76%），是熊血清中的主要磷脂。次要磷脂包括8%的磷脂酰丝氨酸（PS）+磷脂酰肌醇（PI），而磷脂酰乙醇胺（PE）仅占总磷脂的2%。磷脂亚类的比较显示，与黑熊的活跃状态相比，冬眠状态下的PC、PS + PI和SPG显著增加，而PE显著减少（P < 0.05）。这些结果表明，黑熊在冬眠期间磷脂和胆固醇的分解代谢减少，导致它们在冬眠状态下的水平相对于活跃状态有所升高。总之，我们的结果表明，熊在冬眠期间血清胆固醇和磷脂组分（PE除外）增加。据推测，这些脂质的增加可能是由于负责脂质清除的脂蛋白代谢改变所致。
