Maruta Junko, Hashimoto Hironobu, Yamashita Hiroto, Yamashita Hiroyuki, Noguchi Shiro
Department of Pathology, Noguchi Thyroid Clinical and Hospital Foundation, Beppu, Japan.
Diagn Cytopathol. 2003 Jan;28(1):45-8. doi: 10.1002/dc.19999.
Quick cytologic diagnosis by Ultrafast Papanicolaou stain (Ultrafast stain) is useful in various neoplastic diseases; however, it has a significant drawback in that the ground-glass appearance of nuclei is a preparation artifact in some specimens from thyroid aspirations. To eliminate this artifact, the nucleus was stained with Gill-5 Hematoxylin (modified Ultrafast stain) rather than with Richard-Allan Hematoxylin 2 in Ultrafast stain. The applicability of this modified Ultrafast stain for quick diagnosis of thyroid diseases was investigated. Two specimens from each of 251 thyroid aspirations (122 malignant and 131 benign) were prepared using the modified Ultrafast stain and the standard Papanicolaou stain. The sensitivities of cytologic diagnosis in specimens stained by the standard Papanicolaou method and the modified Ultrafast method were 95.0% and 93.3%, respectively, and the specificities were 99.2% and 97.7%, respectively. The modified Ultrafast stain is a reliable procedure for quick diagnosis in thyroid aspiration cytology.
通过超速巴氏染色法（超速染色）进行快速细胞学诊断在各种肿瘤性疾病中很有用；然而，它有一个重大缺点，即细胞核的毛玻璃样外观在一些甲状腺穿刺标本中是制片假象。为消除这种假象，在超速染色中细胞核用吉尔5苏木精（改良超速染色）染色，而不是用理查德-艾伦苏木精2染色。研究了这种改良超速染色在甲状腺疾病快速诊断中的适用性。对251例甲状腺穿刺标本（122例恶性和131例良性）中的每例标本均制备两份，分别采用改良超速染色和标准巴氏染色。标准巴氏染色法和改良超速染色法染色标本的细胞学诊断敏感性分别为95.0%和93.3%，特异性分别为99.2%和97.7%。改良超速染色是甲状腺穿刺细胞学快速诊断的可靠方法。
