Narovlianskiĭ A N, Deriabin P G, Vershinina M Iu, Mezentseva M V, Ershov F I
Vopr Virusol. 2002 Nov-Dec;47(6):17-21.
An experimental model of hepatitis C virus (HCV) infection in cell culture in vitro was used to study the influence of interferon (IFN) inducers on HCV infection activity. In combination with the RT-PCR method, this model was also used to study the dynamics of cytokine mRNA activity for IFN-alpha, IFN-gamma, IL-I beta, IL-2, IL-4, IL-6, IL-8, BL-10, IL-12, IL-18, and TNF-alpha. The research was carried out using long-term cell cultures SW-13 (human paradrenal adenocarcinoma cells) and MT-4 (human cells of lymphoblastoid origin) inoculated with HCV under conditions of acute infection. The obtained data showed that cell cultures SW-13 and MT-4 were sensitive to replication of HCV (cytopathogenic variant). The addition of IFN inducers Savratz, Kagocel, and Cycloferon to infected cell cultures usually resulted in suppression of HCV reproduction in these cultures. Cycloferon had the greatest antiviral activity (virus titer level decreased by a factor of 2.51 g and 5.51 g TCD50 in cell cultures SW-13 and MT-4, respectively). It was suggested that induction of IFN-gamma, IL-4 and IL-8 plays a certain role in HCV reproduction suppression. The results of this work provide an opportunity for more efficacious use of IFN inducers in therapy of HCV infection.
利用丙型肝炎病毒（HCV）体外细胞培养实验模型研究干扰素（IFN）诱导剂对HCV感染活性的影响。结合逆转录聚合酶链反应（RT-PCR）方法，该模型还用于研究IFN-α、IFN-γ、IL-1β、IL-2、IL-4、IL-6、IL-8、BL-10、IL-12、IL-18和TNF-α细胞因子mRNA活性的动态变化。研究采用长期细胞培养的SW-13（人肾上腺皮质腺癌细胞）和MT-4（人淋巴母细胞源性细胞），在急性感染条件下接种HCV。获得的数据表明，细胞培养物SW-13和MT-4对HCV（细胞病变变体）的复制敏感。向感染的细胞培养物中添加IFN诱导剂萨夫拉茨、卡古西尔和环磷酰胺通常会导致这些培养物中HCV繁殖受到抑制。环磷酰胺具有最大的抗病毒活性（在细胞培养物SW-13和MT-4中，病毒滴度水平分别降低了2.51 g和5.51 g TCD50）。研究表明，IFN-γ、IL-4和IL-8的诱导在抑制HCV繁殖中起一定作用。这项工作的结果为在HCV感染治疗中更有效地使用IFN诱导剂提供了机会。
