Gattorno M, Prigione I, Vignola S, Falcini F, Chiesa S, Morandi F, Picco P, Buoncompagni A, Martini A, Pistoia V
2nd Department of Pediatrics, G. Gaslini Scientific Institute for Children and University of Genoa, Largo G. Gaslini 5, 16147, Genoa, Italy.
Clin Exp Rheumatol. 2002 Nov-Dec;20(6):863-6.
CD27 is a member of tumour necrosis factor receptor family. Its expression is predominantly confined to mature lymphocytes and is strongly enhanced after cell activation. Shedding of the CD27 from the surface of activated cells is related to their effector phase. The aim of the present study was to evaluate the levels of soluble CD27 in sera and synovial fluids, together with its expression on circulating and synovial fluid (SF) memory T cells, in children with JIA.
Sera from 40 patients with active JIA were studied for soluble CD27. Paired SF samples were available in 20 patients. Sera from 12 age-matched patients affected with various acute infectious diseases and 12 age-matched healthy subjects were used as controls. In 8 JIA patients freshly isolated circulating and SF lymphocytes were stained for CD27 in CD4+CD45 RO+ T cell subpopulation and analyzed by cytometry.
Soluble CD27 serum levels were significantly higher in patients with polyarticular JIA and acute systemic infectious diseases than in patients with active oligoarticular or healthy controls. Both polyarticular and oligoarticular JIA patients showed increased levels of soluble CD27 in SF when compared with paired serum samples (p = 0.01). In all the patients tested a significant enrichment of CD27- T cells was seen in the SF (median 39.5%, range 18-56%) when compared to paired CD4+CD45RO+ peripheral lymphocytes (median 19.5%, range 5-43%; p = 0.01).
A clear enrichment of CD4+ memory SF T cells with a CD27-phenotype is observed when compared to correspondent circulating T lymphocytes. This issue is conceivably related to re-activation and recruitment of memory T cells to the site of inflammation, and to the subsequent expansion of a subpopulation of "effector" memory T cells.
CD27是肿瘤坏死因子受体家族的成员。其表达主要局限于成熟淋巴细胞，细胞激活后会显著增强。活化细胞表面的CD27脱落与其效应阶段相关。本研究的目的是评估幼年特发性关节炎（JIA）患儿血清和滑液中可溶性CD27的水平，以及其在循环和滑液记忆T细胞上的表达。
对40例活动期JIA患者的血清进行可溶性CD27检测。20例患者有配对的滑液样本。12例年龄匹配的患有各种急性传染病的患者和12例年龄匹配的健康受试者的血清用作对照。对8例JIA患者新鲜分离的循环和滑液淋巴细胞进行CD4+CD45RO+T细胞亚群的CD27染色，并通过流式细胞术进行分析。
多关节型JIA和急性全身感染性疾病患者的可溶性CD27血清水平显著高于活动期少关节型JIA患者或健康对照。与配对血清样本相比，多关节型和少关节型JIA患者的滑液中可溶性CD27水平均升高（p = 0.01）。与配对的CD4+CD45RO+外周淋巴细胞相比（中位数19.5%，范围5 - 43%；p = 0.01），在所有检测患者的滑液中均观察到CD27-T细胞显著富集（中位数39.5%，范围18 - 56%）。
与相应的循环T淋巴细胞相比，观察到具有CD27表型的CD4+记忆性滑液T细胞明显富集。这一现象可能与记忆T细胞重新激活并募集到炎症部位，以及随后“效应”记忆T细胞亚群的扩增有关。
