Hu Wenxue, Li Guanglan, Dong Wei, He Peng, Liu Wei, Wu Yanhua, Liang Huaban, Wen Feng, Yu Feng, Yin Yan, Ye Zhiming, Hao Wenke, Liang Xinling
Department of Nephrology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China.
Institute of Geriatrics, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China.
iScience. 2023 Mar 5;26(4):106336. doi: 10.1016/j.isci.2023.106336. eCollection 2023 Apr 21.
Long-term peritoneal dialysis (PD) is associated with the development of peritoneal fibrosis (PF). Understanding the changes of immune environments and peritoneal mesothelial cells (PMCs) may lead to the discovery of mechanisms of PF. Therefore, we used single-cell RNA sequencing to interrogate cell composition and transcriptome characteristics in dialysate of continuous ambulatory PD (CAPD) patients at different stages. Results showed that six major cell populations were identified in overnight effluent dialysate. Two subsets of macrophages (Macro-c2-SSP1 and Macro-c5-FCN1&SSP1) and PMCs (HSPA1A + PMCs and RPL34 + PMCs) had the property of promoting fibrosis. Long-term patients had higher markers of cytotoxic CD8+T cells. Moreover, the expression levels of fibrosis-related genes were significantly increased and PMCs interacted closely with immune cells in the long-term group (p < 0.05). These data reveal new phenotypes and functional characteristics of immune cells and PMCs in dialysate of CAPD patients with different stages, which provide potential mechanisms and therapeutic strategies for PF.
长期腹膜透析（PD）与腹膜纤维化（PF）的发生有关。了解免疫环境和腹膜间皮细胞（PMC）的变化可能有助于发现PF的机制。因此，我们使用单细胞RNA测序来探究不同阶段持续性非卧床腹膜透析（CAPD）患者透析液中的细胞组成和转录组特征。结果显示，在过夜流出液透析液中鉴定出六个主要细胞群。两个巨噬细胞亚群（Macro-c2-SSP1和Macro-c5-FCN1&SSP1）和PMC（HSPA1A + PMC和RPL34 + PMC）具有促进纤维化的特性。长期患者具有更高的细胞毒性CD8 + T细胞标志物。此外，长期组中纤维化相关基因的表达水平显著增加，且PMC与免疫细胞密切相互作用（p < 0.05）。这些数据揭示了不同阶段CAPD患者透析液中免疫细胞和PMC的新表型和功能特征，为PF提供了潜在机制和治疗策略。
Zotero 插件
