Hale A H, Weber M J
Cell. 1975 Jul;5(3):245-252. doi: 10.1016/0092-8674(75)90099-9.
We have asked whether treatment of normal cultured cells with proteases, other hydrolytic enzymes, or serum can convert them into transient phenocopies of transformed cells with respect to the very high rate of hexose transport characteristic of transformed cells. Treatment of density-inhibited cultures of normal chick embryo fibroblasts with trypsin, plasmin, neuraminidase, or hyaluronidase stimulated their rate of 2-deoxyglucose uptake to a level only marginally higher than that seen in normal exponentially growing cultures, and only 35-45% of that seen in transformed cultures. Addition of the hydrolytic enzymes to growing cell cultures had little effect on 2-deoxyglucose uptake. Serum, however, could stimulate 2-deoxyglucose uptake all the way up to the transformed level. Even though the hydrolases and serum differed in their ability to stimulate 2-deoxyglucose uptake, both reagents were capable of stimulating cell division equally well. Evidence is presented suggesting that the hexose transport rate is controlled by serum factors, and that proteolysis can affect the response of the cells of these factors.
我们曾探讨过，用蛋白酶、其他水解酶或血清处理正常培养的细胞，是否能使其在己糖转运速率方面转变为转化细胞的瞬时表型拷贝，而转化细胞的己糖转运速率非常高。用胰蛋白酶、纤溶酶、神经氨酸酶或透明质酸酶处理正常鸡胚成纤维细胞的密度抑制培养物，会刺激其2-脱氧葡萄糖摄取速率，使其仅略高于正常指数生长培养物中的摄取速率，仅为转化培养物中摄取速率的35%-45%。向生长中的细胞培养物中添加水解酶对2-脱氧葡萄糖摄取影响不大。然而，血清能将2-脱氧葡萄糖摄取刺激至转化水平。尽管水解酶和血清在刺激2-脱氧葡萄糖摄取的能力上有所不同，但两种试剂在刺激细胞分裂方面同样有效。有证据表明，己糖转运速率受血清因子控制，蛋白水解可影响细胞对这些因子的反应。
