Barnes D W, Colowick S P
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5593-7. doi: 10.1073/pnas.74.12.5593.
Evidence is presented that the marked stimulation of sugar uptake and thymidine incorporation by addition of extra Ca2+ to stationary phase mouse 3T3 cells in culture is phosphate dependent and due to the action of the calcium phosphate precipitate formed in the medium. The cells are similarly stimulated by a variety of particulate materials, including calcium pyrophosphate, barium sulfate, kaolin, and polystrene beads. The precipitate effects on sugar uptake are of the same magnitude as those seen with certain hormones (insulin, epidermal growth factor) or with fresh 10% calf serum. The effect of barium sulfate on thymidine incorporation is also of the same magnitude as seen with these hormones, but much less than half that found with fresh calf serum. The stimulation by barium sulfate or hormones of thymidine incorporation is not phosphate dependent.
有证据表明，向培养的静止期小鼠3T3细胞中添加额外的Ca2+后，对糖摄取和胸苷掺入的显著刺激是磷酸盐依赖性的，并且是由于培养基中形成的磷酸钙沉淀的作用。细胞受到多种颗粒物质的类似刺激，包括焦磷酸钙、硫酸钡、高岭土和聚苯乙烯珠。沉淀对糖摄取的影响与某些激素（胰岛素、表皮生长因子）或新鲜10%小牛血清所产生的影响程度相同。硫酸钡对胸苷掺入的影响与这些激素所产生的影响程度也相同，但远小于新鲜小牛血清所产生影响的一半。硫酸钡或激素对胸苷掺入的刺激不是磷酸盐依赖性的。
