[库普弗细胞在严重烫伤大鼠烧伤后肿瘤坏死因子α、白细胞介素-1β和白细胞介素-6产生中的作用]
[The role of Kupffer cells on the postburn production of TNFalpha, IL-1beta and IL-6 in severely scalded rats].
作者信息
Wang Guangyi, Tian Jianguang, Tang Hongtai, Zhu Shihui, Huan Jingning, Ge Shengde, Xia Zhaofan
机构信息
Department of Burns, Chang Hai Hospital, The Second Military Medical University, Shanghai, 200433 P.R. China.
出版信息
Zhonghua Shao Shang Za Zhi. 2002 Oct;18(5):282-4.
OBJECTIVE
To observe the role of Kupffer cells in the postburn production of TNFalpha, IL-1beta and IL-6 in severely scalded rats.
METHODS
(1) The production of TNFalpha, IL-1beta and IL-6 from rat Kupffer cells stimulated by burn serum was observed. (2) The postburn change in the expression of cytokine mRNA from rat Kupffer cells was monitored. (3) The change in the plasma cytokine contents in scalded rats was determined after the application of gadolinium chloride, a specific inhibitor of Kupffer cells.
RESULTS
Kupffer cells could be stimulated by burn serum to release cytokines TNFalpha, IL-1beta and IL-6. The mRNA expression of TNFalpha, IL-1beta and IL-6 from rat Kupffer cells increased significantly after injury. But the postburn plasma levels of TNFalpha, IL-1beta and IL-6 decreased obviously to 34.71%, 36.99% and 33.7% of those in scalding group, respectively, after the Kupffer cell activity was inhibited.
CONCLUSION
The plasma cytokines, i.e. TNFalpha, IL-1beta and IL-6, were primarily produced from Kupffer cells after injury in scalded rats, initiated by TNFalpha, IL-1beta and IL-6 mRNA transcription.
目的
观察库普弗细胞在严重烫伤大鼠烧伤后肿瘤坏死因子α（TNFα）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）产生中的作用。
方法
（1）观察烧伤血清刺激大鼠库普弗细胞后TNFα、IL-1β和IL-6的产生情况。（2）监测大鼠库普弗细胞细胞因子mRNA表达在烧伤后的变化。（3）应用库普弗细胞特异性抑制剂氯化钆后，测定烫伤大鼠血浆细胞因子含量的变化。
结果
烧伤血清可刺激库普弗细胞释放细胞因子TNFα、IL-1β和IL-6。大鼠库普弗细胞TNFα、IL-1β和IL-6的mRNA表达在损伤后显著增加。但抑制库普弗细胞活性后，烧伤后血浆中TNFα、IL-1β和IL-6水平明显降低，分别降至烫伤组的34.71%、36.99%和33.7%。
结论
烫伤大鼠损伤后血浆细胞因子TNFα、IL-1β和IL-6主要由库普弗细胞产生，由TNFα、IL-1β和IL-6 mRNA转录启动。
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