利用基于核酸序列的扩增技术对临床标本中的肠道病毒RNA进行快速检测。
Rapid enterovirus RNA detection in clinical specimens by using nucleic acid sequence-based amplification.
作者信息
Landry Marie L, Garner Robin, Ferguson David
机构信息
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
出版信息
J Clin Microbiol. 2003 Jan;41(1):346-50. doi: 10.1128/JCM.41.1.346-350.2003.
Abstract
Enterovirus (EV) detection by nucleic acid sequence-based amplification was compared with EV isolation in cell culture. The NucliSens Basic kit (bioMerieux) was utilized for RNA detection. For virus isolation, samples were inoculated into MRC-5, primary rhesus monkey kidney, A549, rhabdomyosarcoma, and/or Buffalo green monkey kidney cells.
摘要
通过基于核酸序列扩增的方法检测肠道病毒（EV），并与在细胞培养中分离EV进行比较。使用NucliSens Basic试剂盒（生物梅里埃公司）进行RNA检测。为了分离病毒，将样本接种到MRC-5细胞、原代恒河猴肾细胞、A549细胞、横纹肌肉瘤细胞和/或水牛绿猴肾细胞中。
相似文献
1
Rapid enterovirus RNA detection in clinical specimens by using nucleic acid sequence-based amplification.利用基于核酸序列的扩增技术对临床标本中的肠道病毒RNA进行快速检测。
J Clin Microbiol. 2003 Jan;41(1):346-50. doi: 10.1128/JCM.41.1.346-350.2003.
2
Development, technical performance, and clinical evaluation of a NucliSens basic kit application for detection of enterovirus RNA in cerebrospinal fluid.用于检测脑脊液中肠道病毒RNA的NucliSens基础试剂盒的开发、技术性能及临床评估
J Clin Microbiol. 2005 Jun;43(6):2616-23. doi: 10.1128/JCM.43.6.2616-2623.2005.
3
Detection of enterovirus RNA in cerebrospinal fluid (CSF) using NucliSens EasyQ Enterovirus assay.采用NucliSens EasyQ肠道病毒检测法检测脑脊液（CSF）中的肠道病毒RNA。
J Clin Virol. 2005 Mar;32(3):236-40. doi: 10.1016/j.jcv.2004.08.006.
4
Development and evaluation of nucleic acid sequence based amplification (NASBA) for diagnosis of enterovirus infections using the NucliSens Basic Kit.使用NucliSens Basic试剂盒开发和评估基于核酸序列扩增技术（NASBA）用于肠道病毒感染的诊断。
J Clin Virol. 2002 Feb;24(1-2):117-30. doi: 10.1016/s1386-6532(01)00241-4.
5
Real-time nucleic acid sequence-based amplification using molecular beacons for detection of enterovirus RNA in clinical specimens.使用分子信标进行实时基于核酸序列的扩增以检测临床标本中的肠道病毒RNA
J Clin Microbiol. 2005 Jul;43(7):3136-9. doi: 10.1128/JCM.43.7.3136-3139.2005.
6
Isolation and detection of enterovirus RNA from large-volume water samples by using the NucliSens miniMAG system and real-time nucleic acid sequence-based amplification.使用NucliSens miniMAG系统和基于核酸序列的实时扩增技术从大量水样中分离和检测肠道病毒RNA
Appl Environ Microbiol. 2005 Jul;71(7):3734-40. doi: 10.1128/AEM.71.7.3734-3740.2005.
7
Comparison of the NucliSens Basic kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR assays for rapid detection of enterovirus RNA in clinical specimens.NucliSens Basic试剂盒（基于核酸序列扩增法）与Argene Biosoft肠道病毒通用型逆转录-聚合酶链反应检测法在临床标本中快速检测肠道病毒RNA的比较。
J Clin Microbiol. 2003 Nov;41(11):5006-10. doi: 10.1128/JCM.41.11.5006-5010.2003.
8
Enhanced clinical utility of the NucliSens EasyQ RSV A+B Assay for rapid detection of respiratory syncytial virus in clinical samples.NucliSens EasyQ呼吸道合胞病毒A+B检测法在临床样本中快速检测呼吸道合胞病毒方面增强的临床实用性。
Eur J Clin Microbiol Infect Dis. 2006 Mar;25(3):167-74. doi: 10.1007/S10096-006-0112-4.
9
Detection of rhinoviruses by tissue culture and two independent amplification techniques, nucleic acid sequence-based amplification and reverse transcription-PCR, in children with acute respiratory infections during a winter season.在一个冬季，采用组织培养以及两种独立的扩增技术——基于核酸序列的扩增技术和逆转录-聚合酶链反应，对患有急性呼吸道感染的儿童进行鼻病毒检测。
J Clin Microbiol. 2006 Jan;44(1):166-71. doi: 10.1128/JCM.44.1.166-171.2006.
10
Performance of the GeneXpert enterovirus assay for detection of enteroviral RNA in cerebrospinal fluid.用于检测脑脊液中肠道病毒RNA的GeneXpert肠道病毒检测方法的性能
J Clin Virol. 2008 Sep;43(1):110-3. doi: 10.1016/j.jcv.2008.04.006. Epub 2008 Jun 4.
引用本文的文献
1
Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.用于诊断中枢神经系统感染的脑脊液分子诊断学
Clin Microbiol Rev. 2024 Dec 10;37(4):e0002124. doi: 10.1128/cmr.00021-24. Epub 2024 Oct 15.
2
Echovirus-7 Infection in Children with Unusual Severe Manifestation: A Case Report.伴有异常严重表现的儿童肠道病毒7型感染：一例报告
Infect Chemother. 2021 Mar;53(1):141-145. doi: 10.3947/ic.2020.0081.
3
Detection and Typing of Human Enteroviruses from Clinical Samples by Entire-Capsid Next Generation Sequencing.采用全衣壳高通量测序技术从临床样本中检测和分型人类肠道病毒。
Viruses. 2021 Apr 8;13(4):641. doi: 10.3390/v13040641.
4
Potential Diagnostic Systems for Coronavirus Detection: a Critical Review.用于冠状病毒检测的潜在诊断系统：综述
Biol Proced Online. 2020 Sep 1;22:21. doi: 10.1186/s12575-020-00134-4. eCollection 2020.
5
Biographical Feature: Marie-Louise Landry, M.D.人物特写：玛丽 - 路易丝·兰德里医学博士
J Clin Microbiol. 2019 Oct 23;57(11). doi: 10.1128/JCM.01013-19. Print 2019 Nov.
6
Syndrome Evaluation System for Simultaneous Detection Pathogens Causing Acute Encephalitic Syndrome in India, Part-1: Development and Standardization of the Assay.印度急性脑炎综合征同时检测病原体的综合征评估系统，第1部分：检测方法的开发与标准化
Front Med (Lausanne). 2018 Jul 26;5:208. doi: 10.3389/fmed.2018.00208. eCollection 2018.
7
Laboratory diagnosis of viral infection.病毒感染的实验室诊断
Handb Clin Neurol. 2014;123:123-47. doi: 10.1016/B978-0-444-53488-0.00005-5.
8
Evaluation of LightCycler as a platform for nucleic acid sequence-based amplification (NASBA) in real-time detection of enteroviruses.评估LightCycler作为基于核酸序列扩增（NASBA）平台用于实时检测肠道病毒的性能。
Curr Microbiol. 2008 Jan;56(1):80-3. doi: 10.1007/s00284-007-9043-2. Epub 2007 Oct 2.
9
Comparison of cell culture with RT-PCR for enterovirus detection in stool specimens from patients with acute flaccid paralysis.细胞培养与逆转录聚合酶链反应用于检测急性弛缓性麻痹患者粪便标本中肠道病毒的比较。
J Clin Lab Anal. 2007;21(4):232-6. doi: 10.1002/jcla.20171.
10
Role of cell culture for virus detection in the age of technology.细胞培养在技术时代病毒检测中的作用。
Clin Microbiol Rev. 2007 Jan;20(1):49-78. doi: 10.1128/CMR.00002-06.
本文引用的文献
1
Development and evaluation of nucleic acid sequence based amplification (NASBA) for diagnosis of enterovirus infections using the NucliSens Basic Kit.使用NucliSens Basic试剂盒开发和评估基于核酸序列扩增技术（NASBA）用于肠道病毒感染的诊断。
J Clin Virol. 2002 Feb;24(1-2):117-30. doi: 10.1016/s1386-6532(01)00241-4.
2
Parechoviruses, a novel group of human picornaviruses.细小病毒，一组新型的人类微小核糖核酸病毒。
Ann Med. 2001 Oct;33(7):466-71. doi: 10.3109/07853890109002095.
3
Detection of precytopathic effect of enteroviruses in clinical specimens by centrifugation-enhanced antigen detection.通过离心增强抗原检测法检测临床标本中肠道病毒的细胞病变前效应。
J Clin Microbiol. 2001 Aug;39(8):2755-9. doi: 10.1128/JCM.39.8.2755-2759.2001.
4
Treatment of potentially life-threatening enterovirus infections with pleconaril.用普来可那立治疗潜在危及生命的肠道病毒感染。
Clin Infect Dis. 2001 Jan 15;32(2):228-35. doi: 10.1086/318452.
5
Impact of a diagnostic cerebrospinal fluid enterovirus polymerase chain reaction test on patient management.诊断性脑脊液肠道病毒聚合酶链反应检测对患者管理的影响。
JAMA. 2000;283(20):2680-5. doi: 10.1001/jama.283.20.2680.
6
Reverse-transcription polymerase chain reaction detection of the enteroviruses.肠道病毒的逆转录聚合酶链反应检测
Arch Pathol Lab Med. 1999 Dec;123(12):1161-9. doi: 10.5858/1999-123-1161-RTPCRD.
7
Comparison of rhabdomyosarcoma, buffalo green monkey kidney epithelial, A549 (human lung epithelial) cells and human embryonic lung fibroblasts for isolation of enteroviruses from clinical samples.横纹肌肉瘤、水牛绿猴肾上皮细胞、A549（人肺上皮）细胞与人胚肺成纤维细胞用于从临床样本中分离肠道病毒的比较。
J Clin Virol. 1998 Jul 24;11(1):61-5. doi: 10.1016/s0928-0197(98)00026-9.
8
Evaluation of a commercially available reverse transcription-PCR assay for diagnosis of enteroviral infection in archival and prospectively collected cerebrospinal fluid specimens.评估一种市售逆转录-聚合酶链反应检测法在诊断存档及前瞻性收集的脑脊液标本中肠道病毒感染的应用。
J Clin Microbiol. 1998 Jun;36(6):1741-5. doi: 10.1128/JCM.36.6.1741-1745.1998.
9
Rapid and sensitive detection of enteroviruses in specimens from patients with aseptic meningitis.无菌性脑膜炎患者标本中肠道病毒的快速灵敏检测
J Clin Microbiol. 1996 Jan;34(1):199-201. doi: 10.1128/jcm.34.1.199-201.1996.
10
A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes.一种使用电化学发光（ECL）标记探针的单管定量HIV-1 RNA核酸序列扩增检测法。
J Virol Methods. 1994 Sep;49(2):157-67. doi: 10.1016/0166-0934(94)90040-x.
Zotero 插件