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无菌性脑膜炎患者标本中肠道病毒的快速灵敏检测

Rapid and sensitive detection of enteroviruses in specimens from patients with aseptic meningitis.

作者信息

Yerly S, Gervaix A, Simonet V, Caflisch M, Perrin L, Wunderli W

机构信息

Central Laboratory of Virology, Geneva University Hospital, Switzerland.

出版信息

J Clin Microbiol. 1996 Jan;34(1):199-201. doi: 10.1128/jcm.34.1.199-201.1996.

DOI:10.1128/jcm.34.1.199-201.1996
PMID:8748304
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228761/
Abstract

A 5-h PCR assay (Amplicor enterovirus test) was compared with viral culture for the detection of enteroviruses in cerebrospinal fluid. Of the cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 34% were positive by viral culture whereas 66% were positive by the Amplicor PCR, suggesting that this technique improves the diagnosis of enteroviral meningitis.

摘要

将一种5小时的聚合酶链反应检测法（Amplicor肠道病毒检测法）与病毒培养法进行比较，以检测脑脊液中的肠道病毒。在一次夏季无菌性脑膜炎暴发期间采集的脑脊液标本中，34%通过病毒培养呈阳性，而66%通过Amplicor聚合酶链反应呈阳性，这表明该技术改善了肠道病毒性脑膜炎的诊断。

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本文引用的文献

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Aseptic meningitis in infants < 2 years of age: diagnosis and etiology.2岁以下婴儿的无菌性脑膜炎：诊断与病因
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