无菌性脑膜炎患者标本中肠道病毒的快速灵敏检测
Rapid and sensitive detection of enteroviruses in specimens from patients with aseptic meningitis.
作者信息
Yerly S, Gervaix A, Simonet V, Caflisch M, Perrin L, Wunderli W
机构信息
Central Laboratory of Virology, Geneva University Hospital, Switzerland.
出版信息
J Clin Microbiol. 1996 Jan;34(1):199-201. doi: 10.1128/jcm.34.1.199-201.1996.
Abstract
A 5-h PCR assay (Amplicor enterovirus test) was compared with viral culture for the detection of enteroviruses in cerebrospinal fluid. Of the cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 34% were positive by viral culture whereas 66% were positive by the Amplicor PCR, suggesting that this technique improves the diagnosis of enteroviral meningitis.
摘要
将一种5小时的聚合酶链反应检测法（Amplicor肠道病毒检测法）与病毒培养法进行比较，以检测脑脊液中的肠道病毒。在一次夏季无菌性脑膜炎暴发期间采集的脑脊液标本中，34%通过病毒培养呈阳性，而66%通过Amplicor聚合酶链反应呈阳性，这表明该技术改善了肠道病毒性脑膜炎的诊断。
相似文献
1
Rapid and sensitive detection of enteroviruses in specimens from patients with aseptic meningitis.无菌性脑膜炎患者标本中肠道病毒的快速灵敏检测
J Clin Microbiol. 1996 Jan;34(1):199-201. doi: 10.1128/jcm.34.1.199-201.1996.
2
Evaluation of a commercially available reverse transcription-PCR assay for diagnosis of enteroviral infection in archival and prospectively collected cerebrospinal fluid specimens.评估一种市售逆转录-聚合酶链反应检测法在诊断存档及前瞻性收集的脑脊液标本中肠道病毒感染的应用。
J Clin Microbiol. 1998 Jun;36(6):1741-5. doi: 10.1128/JCM.36.6.1741-1745.1998.
3
Multicenter evaluation of the Amplicor Enterovirus PCR test with cerebrospinal fluid from patients with aseptic meningitis. The European Union Concerted Action on Viral Meningitis and Encephalitis.使用无菌性脑膜炎患者脑脊液对Amplicor肠道病毒聚合酶链反应检测进行多中心评估。欧盟病毒性脑膜炎和脑炎联合行动。
J Clin Microbiol. 1998 Sep;36(9):2652-7. doi: 10.1128/JCM.36.9.2652-2657.1998.
4
Clinical and laboratory detection of nonpolio enteroviruses among different age groups of aseptic meningitis patients in Alexandria, Egypt.埃及亚历山大不同年龄组无菌性脑膜炎患者中非脊髓灰质炎肠道病毒的临床和实验室检测。
J Med Virol. 2021 Jun;93(6):3389-3396. doi: 10.1002/jmv.26480. Epub 2020 Sep 29.
5
Molecular epidemiology of enteroviruses and predominance of echovirus 30 in an Iranian population with aseptic meningitis.肠道病毒的分子流行病学及伊朗无菌性脑膜炎人群中柯萨奇病毒 30 型的优势。
J Neurovirol. 2021 Jun;27(3):444-451. doi: 10.1007/s13365-021-00973-1. Epub 2021 Mar 31.
6
Application of polymerase chain reaction and subsequent phylogenetic analysis to the diagnosis of enteroviral infection in the central nervous system.聚合酶链反应及后续系统发育分析在中枢神经系统肠道病毒感染诊断中的应用。
J Clin Virol. 2002 Jul;25 Suppl 1:S27-38. doi: 10.1016/s1386-6532(02)00031-8.
7
Evaluation of a rapid and completely automated real-time reverse transcriptase PCR assay for diagnosis of enteroviral meningitis.快速且全自动实时逆转录聚合酶链反应检测在病毒性脑膜炎诊断中的应用评价。
J Clin Microbiol. 2011 Feb;49(2):528-33. doi: 10.1128/JCM.01570-10. Epub 2010 Dec 15.
8
Multicenter evaluating of a commercially available PCR assay for diagnosing enterovirus infection in a panel of cerebrospinal fluid specimens.一项针对一组脑脊液标本中肠道病毒感染诊断的市售聚合酶链反应检测法的多中心评估。
J Clin Microbiol. 1996 Dec;34(12):3002-6. doi: 10.1128/jcm.34.12.3002-3006.1996.
9
Detection and typing of enteroviruses from CSF specimens from patients diagnosed with meningitis/encephalitis.从诊断为脑膜炎/脑炎患者的脑脊液标本中检测肠道病毒并进行分型。
J Clin Virol. 2008 Oct;43(2):207-11. doi: 10.1016/j.jcv.2008.06.016. Epub 2008 Aug 9.
10
Molecular typing and epidemiology of enteroviruses identified from an outbreak of aseptic meningitis in Belgium during the summer of 2000.2000年夏季比利时无菌性脑膜炎暴发中鉴定出的肠道病毒的分子分型及流行病学研究
J Med Virol. 2003 Jul;70(3):420-9. doi: 10.1002/jmv.10412.
引用本文的文献
1
Identification of a Conserved, Linear Epitope on VP3 of Species Recognized by a Broad-Spectrum Monoclonal Antibody.鉴定一种广谱单克隆抗体识别的 种属 VP3 上的保守线性表位。
Viruses. 2023 Apr 21;15(4):1028. doi: 10.3390/v15041028.
2
Retrospective analysis of multiplex polymerase chain reaction-based molecular diagnostics (SES) in 70 patients with suspected central nervous system infections: A single-center study.基于多重聚合酶链反应的分子诊断（SES）在70例疑似中枢神经系统感染患者中的回顾性分析：一项单中心研究。
Ann Indian Acad Neurol. 2016 Oct-Dec;19(4):482-490. doi: 10.4103/0972-2327.192483.
3
Enterovirus and parechovirus infection in children: a brief overview.儿童肠道病毒和细小病毒感染：简要概述
Eur J Pediatr. 2016 Aug;175(8):1023-9. doi: 10.1007/s00431-016-2725-7. Epub 2016 May 7.
4
Enteroviral central nervous system infections in children of the region of monastir, Tunisia: diagnosis, laboratory findings of cerebrospinal fluid and clinical manifestations.突尼斯莫纳斯提尔地区儿童肠道病毒中枢神经系统感染：诊断、脑脊液实验室检查结果及临床表现
Indian J Virol. 2012 Dec;23(3):294-302. doi: 10.1007/s13337-012-0104-1. Epub 2012 Sep 4.
5
Evaluation of different clinical sample types in diagnosis of human enterovirus 71-associated hand-foot-and-mouth disease.不同临床样本类型在人类肠道病毒71型相关手足口病诊断中的评估
J Clin Microbiol. 2007 Jun;45(6):1858-66. doi: 10.1128/JCM.01394-06. Epub 2007 Apr 19.
6
Development, technical performance, and clinical evaluation of a NucliSens basic kit application for detection of enterovirus RNA in cerebrospinal fluid.用于检测脑脊液中肠道病毒RNA的NucliSens基础试剂盒的开发、技术性能及临床评估
J Clin Microbiol. 2005 Jun;43(6):2616-23. doi: 10.1128/JCM.43.6.2616-2623.2005.
7
New reverse transcription-PCR assay for rapid and sensitive detection of enterovirus genomes in cerebrospinal fluid specimens of patients with aseptic meningitis.用于快速灵敏检测无菌性脑膜炎患者脑脊液标本中肠道病毒基因组的新型逆转录-聚合酶链反应检测法。
J Clin Microbiol. 2003 Dec;41(12):5726-8. doi: 10.1128/JCM.41.12.5726-5728.2003.
8
Comparison of the NucliSens Basic kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR assays for rapid detection of enterovirus RNA in clinical specimens.NucliSens Basic试剂盒（基于核酸序列扩增法）与Argene Biosoft肠道病毒通用型逆转录-聚合酶链反应检测法在临床标本中快速检测肠道病毒RNA的比较。
J Clin Microbiol. 2003 Nov;41(11):5006-10. doi: 10.1128/JCM.41.11.5006-5010.2003.
9
Molecular analysis of cerebrospinal fluid in viral diseases of the central nervous system.中枢神经系统病毒性疾病中脑脊液的分子分析。
J Clin Virol. 2003 Jan;26(1):1-28. doi: 10.1016/s1386-6532(02)00173-7.
10
Diagnosis of enterovirus infection by genus-specific PCR and enzyme-linked immunosorbent assays.通过属特异性聚合酶链反应和酶联免疫吸附测定法诊断肠道病毒感染。
J Clin Microbiol. 2003 Feb;41(2):841-4. doi: 10.1128/JCM.41.2.841-844.2003.
本文引用的文献
1
Aseptic meningitis in infants < 2 years of age: diagnosis and etiology.2岁以下婴儿的无菌性脑膜炎：诊断与病因
J Infect Dis. 1993 Oct;168(4):888-92. doi: 10.1093/infdis/168.4.888.
2
Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens.通过磁珠提取和聚合酶链反应检测临床标本中的肠道病毒RNA对肠道病毒感染进行快速诊断。
J Clin Microbiol. 1993 Jan;31(1):31-8. doi: 10.1128/jcm.31.1.31-38.1993.
3
Detection of enteroviral RNA by polymerase chain reaction in cerebrospinal fluid from patients with aseptic meningitis.通过聚合酶链反应检测无菌性脑膜炎患者脑脊液中的肠道病毒RNA。
Scand J Infect Dis. 1993;25(5):547-57. doi: 10.3109/00365549309008542.
4
Diagnosis of enteroviral central nervous system infection by polymerase chain reaction during a large community outbreak.在一次大规模社区疫情期间通过聚合酶链反应诊断肠道病毒中枢神经系统感染
Pediatr Infect Dis J. 1994 Mar;13(3):177-82. doi: 10.1097/00006454-199403000-00002.
5
Enteroviral meningitis in infancy: potential role for polymerase chain reaction in patient management.婴儿肠道病毒性脑膜炎：聚合酶链反应在患者管理中的潜在作用。
Pediatrics. 1994 Aug;94(2 Pt 1):157-62.
6
Detection of enteroviruses in cerebrospinal fluids: enzymatic amplification and hybridization with a biotinylated riboprobe.脑脊液中肠道病毒的检测：酶促扩增及与生物素化核糖探针的杂交
Mol Cell Probes. 1994 Feb;8(1):15-22. doi: 10.1006/mcpr.1994.1003.
7
Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.采用比色微孔检测法的聚合酶链反应诊断肠道病毒脑膜炎。
J Clin Microbiol. 1994 Oct;32(10):2590-2. doi: 10.1128/jcm.32.10.2590-2592.1994.
8
Management of central nervous system infections during an epidemic of enteroviral aseptic meningitis.肠道病毒性无菌性脑膜炎流行期间中枢神经系统感染的管理
J Pediatr. 1980 Mar;96(3 Pt 2):559-63. doi: 10.1016/s0022-3476(80)80866-3.
9
Enteroviral meningitis in early infancy: significance in community outbreaks.
Pediatr Infect Dis. 1982 May-Jun;1(3):151-4. doi: 10.1097/00006454-198205000-00004.
10
Enteroviruses and meningitis.肠道病毒与脑膜炎
Pediatr Infect Dis. 1983 Jul-Aug;2(4):333-41. doi: 10.1097/00006454-198307000-00019.
Zotero 插件