Schmit J C, Brody S
J Bacteriol. 1975 Jun;122(3):1071-5. doi: 10.1128/jb.122.3.1071-1075.1975.
The glucosamine and galactosamine content of mycelia was measured in cultures of Neurospora crassa grown on the surface of dialysis membranes. The glucosamine content was relatively constant throughout the different regions of the mycelial mat. The galactosamine content, however, was always lower in the growing-front region of the mycelial mat than in the older regions. At most, only low levels of galactosamine were necessary for the formation of hyphae at the growing front of a mycelial mat. Thus, galactosamine-containing polymers cannot be a major shape-determining component of the cell walls of these hyphae in Neurospora. The effect of conidiation on the amino sugar content was determined by using the bd (band) strain of N. crassa. When grown on the surface of dialysis membranes, this strain rhythmically produced regions of conidiating and non-conidiating growth. With this strain, it was concluded that conidiation did not affect the amino sugar levels. Since conidia that contained only very low levels of galactosamine were produced from regions of the mycelial mat that contained much higher levels of this amino sugar, there must be some mechanism of spatial differentiation that prevented the accumulation of galactosamine-containing polymers in conidia.
在透析膜表面生长的粗糙脉孢菌培养物中，测定了菌丝体的葡萄糖胺和半乳糖胺含量。在菌丝垫的不同区域，葡萄糖胺含量相对恒定。然而，在菌丝垫的生长前沿区域，半乳糖胺含量总是低于较老的区域。在菌丝垫生长前沿形成菌丝时，最多只需要低水平的半乳糖胺。因此，含半乳糖胺的聚合物不可能是粗糙脉孢菌中这些菌丝细胞壁的主要形状决定成分。通过使用粗糙脉孢菌的bd（带）菌株，确定了产孢对氨基糖含量的影响。当在透析膜表面生长时，该菌株有节奏地产生产孢和不产孢生长的区域。通过该菌株得出结论，产孢不影响氨基糖水平。由于从菌丝垫中含有高得多水平这种氨基糖的区域产生的分生孢子只含有非常低水平的半乳糖胺，所以一定存在某种空间分化机制，阻止含半乳糖胺的聚合物在分生孢子中积累。
