[反相高效液相色谱法测定忍冬中忍冬苷A和C]
[Determination of loniceroside A and C in Lonicerae japonicae by RP-HPLC].
作者信息
Zhang Xiao-Guang, Wang Zhi-Min, Song Xiao-Mei, Zhu Jing-Jing, Gao Hui-Min, Lin Li-Mei
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Oct;32(20):2149-51, 2210.
OBJECTIVE
To develop an HPLC method for determination of loniceroside A and loniceroside C in Lonicerae japonicae.
METHOD
The analysis was carried out on an Alltech C18 column (4.6 mm x 250 mm, 5 microm) evaluated with methanol-acetonitrile-0.1% glacial acetic acid as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 210 nm for UV detection.
RESULT
The calibration curves were linear over the range from 0.15 to 2.25 microg (r = 0.999 9) for loniceroside A and 0.11 to 1.65 microg (r = 0.999 1) for loniceroside C. The average recoveries were 99.9% and 98.3%, respectively.
CONCLUSION
The contents of Loniceroside A&C are diverse in Flos Lonicerae japonicae in different regions.
目的
建立一种用于测定忍冬中忍冬苷A和忍冬苷C的高效液相色谱法。
方法
采用Alltech C18柱（4.6 mm×250 mm，5μm）进行分析，以甲醇 - 乙腈 - 0.1%冰醋酸为流动相。流速为1.0 mL·min⁻¹，紫外检测波长为210 nm。
结果
忍冬苷A的校准曲线在0.15至2.25μg范围内呈线性（r = 0.999 9），忍冬苷C的校准曲线在0.11至1.65μg范围内呈线性（r = 0.999 1）。平均回收率分别为99.9%和98.3%。
结论
不同地区忍冬花中忍冬苷A和忍冬苷C的含量存在差异。
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