[高效液相色谱法测定酸枣中酸枣仁皂苷II的含量]
[Determination of zizybeoside II of Ziziphus jujuba by HPLC].
作者信息
Niu Ji-Wei, Zhang Qi-Wei, Gong Mu-Xin, Yan Li-Hua, Zhu Jing-Jing, Wang Zhi-Min, Li Qin-Fan
机构信息
College of Veterinary Medicine, Northwest Agricultural and Forest University, Yangling 712100, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Dec;33(24):2935-7.
OBJECTIVE
To develop a quantitative method for determination of zizybeoside II in Ziziphus jujuba.
METHOD
The samples were separated at 30 degrees C on a Zorbax SB-C18 column eluted with methanol-water (20 : 80) as the mobile phase. Flow rate was set at 1.0 mL x min(-1) and the detection wavelength was set at 210 nm.
RESULT
The calibration curve was linear within the range from 0.046 to 0.582 microg (r = 0.999 9) and the average recovery was 97.2%. 12 batches of the crude drugs purchased from different areas were determined and the contents of zizybeoside II in Fructus Jujubae were fluctuated from 0.013% to 0.041%.
CONCLUSION
The method is simple, repeatable and could be used for the quality control of Z. jujuba.
目的
建立一种测定酸枣中酸枣仁皂苷II含量的定量方法。
方法
样品在30℃下于Zorbax SB-C18柱上分离，以甲醇-水（20∶80）为流动相进行洗脱。流速设定为1.0 mL·min⁻¹，检测波长设定为210 nm。
结果
校准曲线在0.046至0.582 μg范围内呈线性（r = 0.999 9），平均回收率为97.2%。对从不同地区购买的12批药材进行测定，酸枣仁中酸枣仁皂苷II的含量在0.013%至0.041%之间波动。
结论
该方法简便、可重复，可用于酸枣的质量控制。
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