Bao X F, Fang J N
Shanghai Institute of Materia Medica, Academia Sinica, Shanghai 200031, China.
Zhongguo Zhong Yao Za Zhi. 2001 May;26(5):326-8.
To compare the release ability of water-soluble polysaccharides in sporoderm-broken and nonbroken spores of Ganoderma lucidum, and establish a comparatively correct method for the determination and analysis of polysaccharide contents in Chinese herbs.
The release ability of water-soluble polysaccharides was determined on the basis of phenol-sulfuric acid modification in different conditions.
The release ability of polysaccharides of sporoderm-broken spores was much greater than that of nonbroken spores; and the phenol-sulfuric acid modified cation method proved excellent in accuracy and reproducibility, with a relative error less than 1.5%.
The spores should be wall-wracked if used as a nutriment, or for extraction and analysis of their effective components. The method can be successfully used for the determination of polysaccharide contents in Chinese herbs or nutriments.
比较破壁与未破壁灵芝孢子中水溶性多糖的释放能力，建立较为准确的中药材多糖含量测定与分析方法。
在不同条件下，基于苯酚-硫酸法测定水溶性多糖的释放能力。
破壁孢子的多糖释放能力远大于未破壁孢子；苯酚-硫酸改良阳离子法在准确性和重现性方面表现出色，相对误差小于1.5%。
若将孢子用作营养剂，或用于提取和分析其有效成分，应进行破壁处理。该方法可成功用于中药材或营养剂中多糖含量的测定。
