Glycobiology and Glycochemistry Lab., Shanghai Institute of Materia Medica, Chinese Academy of Sciences and Infinitus, Shanghai 201203, China.
Carbohydr Res. 2012 May 15;353:100-5. doi: 10.1016/j.carres.2012.02.029. Epub 2012 Mar 6.
Ganoderma lucidum is an edible and medicinal mushroom used widely in East Asia. In recent years, its spores have been used as a supplement in combination with other forms of antitumor therapies. The cell wall of Ganoderma lucidum spores contains a high amount of polysaccharides. In this study, a neutral polysaccharide, GLSA50-1B, was isolated from sporoderm-broken spores of Ganoderma lucidum, by hot-water extraction, graded ethanol precipitation, anion-exchange chromatography, and gel permeation chromatography. Using sugar compositional analysis, methylation analysis, partial acid hydrolysis, acetolysis, and NMR and ESI-MS spectroscopy, GLSA50-1B was elucidated to be a novel β-D-glucan featured by a 1,6-linked β-D-Glcp backbone with different length of branches consisting of terminal and 1,4-linked Glcp residues, attached to O-4 of alternative Glc residues in the backbone.
灵芝是一种在东亚广泛使用的食用和药用蘑菇。近年来，其孢子已被用作与其他形式的抗肿瘤疗法联合使用的补充剂。灵芝孢子的细胞壁含有大量多糖。在这项研究中，通过热水提取、分级乙醇沉淀、阴离子交换色谱和凝胶渗透色谱，从灵芝破壁孢子中分离到一种中性多糖 GLSA50-1B。通过糖组成分析、甲基化分析、部分酸水解、乙酰解和 NMR 和 ESI-MS 光谱，GLSA50-1B 被阐明为一种新型的β-D-葡聚糖，其特征是具有 1,6 连接的β-D-Glcp 主链，不同长度的支链由末端和 1,4 连接的 Glcp 残基组成，连接到主链中交替的 Glc 残基的 O-4 上。
