Xu Y, Fu M, Xu Y, Liu B
Apolipoprotein Research Laboratory, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Hua Xi Yi Ke Da Xue Xue Bao. 2001 Dec;32(4):509-12.
To investigate the change of the contents of serum HDL subclasses in obese subjects.
Contents of serum HDL subclasses in healthy controls(n = 64) and obese subjects(n = 58) were determined by two-dimensional gel electrophoresis associated with immunodetection method.
The pre-beta 1 HDL, HDL3c and HDL3b contents increased significantly (P < 0.001) and the HDL2b content decreased significantly (P < 0.001) in obese subjects as compared with those in healthy controls. In male obese subjects, the pre-beta 1 HDL and HDL3c levels were significantly higher(P < 0.01 and P < 0.05, respectively) and HDL2b level was significantly lower(P < 0.05), compared with those in female obese subjects. In male control subjects, the level of pre-beta 1 HDL was significantly higher than that in female subjects. In obese subjects, the concentrations of BMI and serum TG had positive correlation with those of pre-beta 1 HDL(r = 0.382, P < 0.001), HDL3c(r = 0.232, P < 0.05) and HDL3b(r = 0.294, P < 0.01) and negative correlation with that of HDL2b(r = -0.304, P < 0.01).
The particle size of HDL in obese subjects showed a general shift toward smaller size, which indicated that the maturation of HDL could be abnormal in obese subjects. The particle size of HDL was found to be smaller in both obese and control male subjects than in female subjects, suggesting that sex seems to be related to the contents of serum HDL subclasses.
研究肥胖受试者血清高密度脂蛋白（HDL）亚类含量的变化。
采用二维凝胶电泳结合免疫检测法测定64名健康对照者和58名肥胖受试者血清HDL亚类的含量。
与健康对照者相比，肥胖受试者前β1-HDL、HDL3c和HDL3b含量显著升高（P<0.001），HDL2b含量显著降低（P<0.001）。男性肥胖受试者的前β1-HDL和HDL3c水平显著高于女性肥胖受试者（分别为P<0.01和P<0.05），HDL2b水平显著低于女性肥胖受试者（P<0.05）。男性对照者的前β1-HDL水平显著高于女性对照者。在肥胖受试者中，体重指数（BMI）和血清甘油三酯（TG）浓度与前β1-HDL（r=0.382，P<0.001）、HDL3c（r=0.232，P<0.05）和HDL3b（r=0.294，P<0.01）浓度呈正相关，与HDL2b浓度呈负相关（r=-0.304，P<0.01）。
肥胖受试者HDL颗粒大小总体上向较小尺寸偏移，提示肥胖受试者HDL成熟可能异常。肥胖男性和对照男性受试者的HDL颗粒大小均小于女性受试者，提示性别似乎与血清HDL亚类含量有关。
