Tian Li, Jia Lianqun, Mingde Fu, Tian Ying, Xu Yanhua, Tian Haoming, Yang Yuye
Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
Lipids. 2006 Aug;41(8):789-96. doi: 10.1007/s11745-006-5032-7.
The object of this study was to investigate the characteristics of lipid metabolism in obese subjects, with particular emphasis on the alteration of HDL subclass contents and distributions. A population of 581 Chinese individuals was divided into four groups (25 underweight subjects, 288 of desirable weight, 187 overweight, and 45 obese) according to body mass index (BMI). Apoprotein A-I (apoA-I) contents of plasma HDL sub-classes were determined by 2-D gel electrophoresis associated with an immunodetection method. The concentrations of TG and the apoA-I content of pre-beta 1-HDL were significantly higher (P < 0.01 and P < 0.01, respectively), but the levels of HDL cholesterol, and the apoA-I contents of HDL2a and HDL2b were significantly lower (P < 0.01, P < 0.05, and P < 0.01, respectively) in obese subjects than in subjects having a desirable weight. Moreover, with the elevation of BMI, small-sized pre-beta 1-HDL increased gradually and significantly, whereas large-sized HDL2b decreased gradually and significantly. Meanwhile, the variations in HDL subclass distribution were more obvious with the elevation of TG levels in obese as well as overweight subjects. In addition, Pearson correlation analysis revealed that BMI and TG levels were positively correlated with pre-beta 1-HDL but negatively correlated with HDL2b. Multiple regression analysis also showed that TG concentrations were associated independently and positively with high pre-beta 1-HDL and independently and negatively with low HDL2b in obese and overweight subjects. The HDL particle size was smaller in obese and overweight subjects. The shift to smaller size was more obvious with the elevation of BMI and TG, especially TG levels. These observations, in turn, indicated that HDL maturation might be abnormal, and reverse cholesterol transport might be impaired.
本研究的目的是调查肥胖受试者的脂质代谢特征，尤其着重于高密度脂蛋白（HDL）亚类含量及分布的变化。根据体重指数（BMI），将581名中国个体分为四组（25名体重过轻受试者、288名体重正常者、187名超重者和45名肥胖者）。采用二维凝胶电泳结合免疫检测法测定血浆HDL亚类中的载脂蛋白A-I（apoA-I）含量。肥胖受试者的甘油三酯（TG）浓度和前β1-HDL的apoA-I含量显著更高（分别为P < 0.01和P < 0.01），但肥胖受试者的HDL胆固醇水平以及HDL2a和HDL2b的apoA-I含量显著更低（分别为P < 0.01、P < 0.05和P < 0.01），与体重正常的受试者相比。此外，随着BMI升高，小尺寸的前β1-HDL逐渐且显著增加，而大尺寸的HDL2b逐渐且显著减少。同时，在肥胖及超重受试者中，随着TG水平升高，HDL亚类分布的变化更为明显。另外，Pearson相关性分析显示，BMI和TG水平与前β1-HDL呈正相关，但与HDL2b呈负相关。多元回归分析还表明，在肥胖和超重受试者中，TG浓度与高水平的前β1-HDL独立且呈正相关，与低水平的HDL2b独立且呈负相关。肥胖和超重受试者的HDL颗粒尺寸更小。随着BMI和TG升高，尤其是TG水平升高，向更小尺寸的转变更为明显。这些观察结果进而表明，HDL成熟可能异常，胆固醇逆向转运可能受损。
