Lynch W E, Short J, Lieberman I
Cancer Res. 1976 Mar;36(3):901-4.
Just as after 70% hepatectomy, the activity of the 7.1 S DNA polymerase, but not the 3.2 S polymerase, is elevated in liver nuclei from unoperated animals in which hepatic DNA replication has been induced with a mixture of biochemicals or by a dietary manipulation. Again as with regenerating liver, the stimulated intact livers show a relationship between the increases in the enzyme activity and thymidine incorporation in vivo over a wide range of hepatic responses. These observations are consistent with a role for the 7.1 S activity in nuclear DNA replication. Cytosine arabinoside 5'-triphosphate and novobiocin can be used to distinguish between the 3.2 S and 7.1 S polymerases from nuclei of stimulated intact liver as well as of regenerating liver.
正如在70%肝切除术后一样，在用生化物质混合物或通过饮食调控诱导肝脏DNA复制的未手术动物的肝细胞核中，7.1S DNA聚合酶的活性升高，而3.2S聚合酶的活性未升高。同样与再生肝脏的情况一样，受到刺激的完整肝脏在广泛的肝脏反应范围内，显示出酶活性增加与体内胸苷掺入之间的关系。这些观察结果与7.1S活性在核DNA复制中的作用一致。5'-三磷酸阿糖胞苷和新生霉素可用于区分来自受刺激的完整肝脏以及再生肝脏细胞核中的3.2S和7.1S聚合酶。
