Mansilla María C, Aguilar Pablo S, Albanesi Daniela, Cybulski Larisa E, Altabe Silvia, de Mendoza Diego
Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Instituto de Biología Molecular y Celular de Rosario, Universidad Nacional de Rosario, 2000 Rosario, Argentina.
Prostaglandins Leukot Essent Fatty Acids. 2003 Feb;68(2):187-90. doi: 10.1016/s0952-3278(02)00269-7.
The Des pathway of Bacillus subtilis regulates the expression of the acyl-lipid desaturase, Des, thereby controlling the synthesis of unsaturated fatty acids from saturated phospholipid precursors. Activation of this pathway takes place when cells are shifted to low growth temperature or when they are grown in minimal media in the absence of isoleucine supplies. The master switch for the Des pathway is a two-component regulatory system composed of a membrane-associated kinase, DesK, and a soluble transcriptional regulator, DesR, which stringently controls transcription of the des gene. We propose that both, a decrease in membrane fluidity at constant temperature and a temperature downshift induce des by the same mechanism, involving the ability of DesK to sense a decrease in membrane fluidity.
枯草芽孢杆菌的Des途径调节酰基脂质去饱和酶Des的表达，从而控制从饱和磷脂前体合成不饱和脂肪酸。当细胞转移到低生长温度或在缺乏异亮氨酸供应的基本培养基中生长时，该途径被激活。Des途径的主开关是一个双组分调节系统，由膜相关激酶DesK和可溶性转录调节因子DesR组成，它们严格控制des基因的转录。我们提出，在恒定温度下膜流动性的降低和温度下降通过相同的机制诱导des，这涉及DesK感知膜流动性降低的能力。
