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人垂体生长激素：一种具有生物活性的一百二十一肽片段，对应于激素分子中的氨基酸残基15 - 125。

Human pituitary growth hormone: a biologically active hendekakaihekaton peptide fragment corresponding to amino-acid residues 15-125 in the hormone molecule.

作者信息

Li C H

出版信息

Proc Natl Acad Sci U S A. 1975 Oct;72(10):3878-82. doi: 10.1073/pnas.72.10.3878.

DOI:10.1073/pnas.72.10.3878
PMID:812085
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433099/
Abstract

A hendekakaihekaton peptide fragment has been prepared by cyanogen bromide cleavage of the NH2-terminal 134-residue fragment of human pituitary growth hormone. It was characterized by amino-acid and end-group analyses, exclusion chromatography, disc electrophoresis, circular dichroism, and ultracentrifugation. The fragment, corresponding to amino-acid residues 15-125 in the hormone molecule, possesses hepatic ornithine decarboxylase (EC 4.1.1.17; L-ornithine carboxy-lyase) stimulating, lactogenic, and somatotrophic activity. It has immunoreactivity in the microcomplement-fixation and radioimmunoassay experiments. The circular dichroism data indicate that the hendekakaihekaton peptide fragment is devoid of secondary and tertiary structure.

摘要

通过溴化氰裂解人垂体生长激素的NH2末端134个残基片段制备了一个十一肽片段。通过氨基酸和端基分析、排阻色谱、圆盘电泳、圆二色性和超速离心对其进行了表征。该片段对应于激素分子中的15-125位氨基酸残基，具有刺激肝脏鸟氨酸脱羧酶（EC 4.1.1.17；L-鸟氨酸羧基裂解酶）、催乳和促生长活性。在微量补体结合和放射免疫测定实验中具有免疫反应性。圆二色性数据表明该十一肽片段缺乏二级和三级结构。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5892/433099/7d40eba7d04d/pnas00053-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5892/433099/7d40eba7d04d/pnas00053-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5892/433099/7d40eba7d04d/pnas00053-0099-a.jpg

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Human pituitary growth hormone: a biologically active hendekakaihekaton peptide fragment corresponding to amino-acid residues 15-125 in the hormone molecule.人垂体生长激素：一种具有生物活性的一百二十一肽片段，对应于激素分子中的氨基酸残基15 - 125。
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3878-82. doi: 10.1073/pnas.72.10.3878.
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Bioassay of hypophyseal growth hormone; the tibia test.垂体生长激素的生物测定；胫骨试验。
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Proc Natl Acad Sci U S A. 1976 May;73(5):1476-9. doi: 10.1073/pnas.73.5.1476.
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Human pituitary growth hormone. VI. Modified procedure of isolation and NH2-terminal amino acid sequence.人垂体生长激素。VI. 改进的分离方法及氨基末端氨基酸序列
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Nonenzymatic cleavage of peptide bonds: the methionine residues in bovine pancreatic ribonuclease.肽键的非酶促裂解：牛胰核糖核酸酶中的甲硫氨酸残基
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Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition.定量微量补体结合及其在通过特异性抗原-抗体抑制研究抗原结构中的应用。
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Properties of and structural investigations on growth hormones isolated from bovine, monkey and human pituitary glands.
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Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion.凝胶中的抗原-抗体反应。IV. 扩散协调系统中的反应类型。
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Plasma growth hormone concentration in the rat determined by radioimmunoassay: influence of sex, pregnancy, lactation, anesthesia, hypophysectomy and extrasellar pituitary transplants.用放射免疫分析法测定大鼠血浆生长激素浓度：性别、妊娠、哺乳、麻醉、垂体切除及垂体鞍外移植的影响。
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