Stability studies of ubiquinol in plasma.

作者信息

Hectors M P C, van Tits L J H, de Rijke Y B, Demacker P N M

机构信息

Laboratory of General Internal Medicine, 564 AKC, University Medical Center, Nijmegen, 6500 HB Nijmegen, The Netherlands.

出版信息

Ann Clin Biochem. 2003 Jan;40(Pt 1):100-1. doi: 10.1258/000456303321016240.

DOI:10.1258/000456303321016240

PMID:12542918

Abstract

BACKGROUND

Ubiquinol is a sensitive redox marker in the first line of the antioxidative defence mechanism and is increasingly being measured in oxidation studies. Because of its apparent instability during storage and processing, we compared various storage conditions.

METHOD

Blood was collected from three volunteers into tubes containing EDTA; it was then separated at 4 degrees C and cryopreserved with saccharose (final concentration 6 g/L). Aliquots were stored with or without glutathione or butylated hydroxytoluene at -20 degrees C and -80 degrees C.

RESULTS AND CONCLUSION

Ubiquinol in samples stored at -20 degrees C was not stable; however, it was stable when stored at -80 degrees C, even without addition of antioxidant. By contrast, alpha-tocopherol was stable under all conditions studied.

摘要