I型胶原C端肽（CTX）的血清和血浆片段在低温储存3年期间保持稳定。
Serum and plasma fragments of C-telopeptides of type I collagen (CTX) are stable during storage at low temperatures for 3 years.
作者信息
Qvist P, Munk M, Hoyle N, Christiansen C
机构信息
Nordic Bioscience A/S, Herlev Hovedgade 207, DK-2730 Herlev, Denmark.
出版信息
Clin Chim Acta. 2004 Dec;350(1-2):167-73. doi: 10.1016/j.cccn.2004.07.024.
BACKGROUND
Control of pre-analytical variables is essential for successful application of biological markers, including bone resorption markers, in clinical trials and routine use. The effect of storage temperature on stability of bone resorption markers have not been subject of systematically investigation, and therefore the present study was set out to determine the stability of C-telopeptides of type I collagen (CTX) in serum and plasma samples stored frozen for 3 years.
METHODS
The serum and plasma levels of CTX were determined in samples aliquoted and stored frozen for up to 3 years.
RESULTS
No significant decrease could be detected in neither serum nor plasma samples after 3 years of storage at -20, -80 or -150 degrees C. However, at elevated temperature, i.e. 4 and 37 degrees C, improved stability of CTX was observed in EDTA plasma samples compared to serum.
CONCLUSIONS
CTX is stable in frozen serum and plasma samples for up to 3 years. EDTA plasma might be the preferred matrix due to improved stability at elevated temperatures.
背景
在临床试验和日常应用中，控制分析前变量对于成功应用生物标志物（包括骨吸收标志物）至关重要。储存温度对骨吸收标志物稳定性的影响尚未得到系统研究，因此本研究旨在确定在 -20℃、-80℃或 -150℃冷冻储存3年的血清和血浆样本中I型胶原C末端肽（CTX）的稳定性。
方法
对分装并冷冻储存长达3年的样本测定CTX的血清和血浆水平。
结果
在 -20℃、-80℃或 -150℃储存3年后，血清和血浆样本中均未检测到显著下降。然而，在较高温度下，即4℃和37℃时，与血清相比，EDTA血浆样本中CTX的稳定性有所提高。
结论
CTX在冷冻的血清和血浆样本中可稳定保存长达3年。由于在较高温度下稳定性提高，EDTA血浆可能是首选基质。
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