外源性晚期糖基化终产物诱导正常大鼠出现糖尿病样血管功能障碍：糖尿病视网膜病变的一个因素。

Exogenous advanced glycosylation end products induce diabetes-like vascular dysfunction in normal rats: a factor in diabetic retinopathy.

作者信息

Xu Xun, Li Zhiping, Luo Dawei, Huang Yufeng, Zhu Jianfeng, Wang Xiaojue, Hu Honghui, Patrick C P

机构信息

Department of Ophthalmology, Shanghai First People's Hospital, Shanghai, China 200080.

出版信息

Graefes Arch Clin Exp Ophthalmol. 2003 Jan;241(1):56-62. doi: 10.1007/s00417-002-0575-7. Epub 2002 Dec 4.

DOI:10.1007/s00417-002-0575-7

PMID:12545293

Abstract

BACKGROUND

Diabetic retinopathy has been shown to be directly associated with the degree and duration of hyperglycemia, and advanced glycosylation end products (AGEs) have been implicated in this pathological process. The purpose of the experiments reported here was to study the effect of AGE deposition on retinal vascular damage which leads to diabetic retinopathy.

METHODS

Intravenous injection of exogenous AGEs was used to treat wild-type non-diabetic Sprague-Dawley rats. One of the two retinal slides from each animal was treated using immunohistochemical staining to label retinal vascular AGE deposition, the other H&E staining for counting of capillary pericytes. The results were compared with the findings in untreated wild-type and diabetic controls and in rats treated with unmodified rat serum albumin (RSA).

RESULTS

After 2 weeks of continuous treatment, AGEs were identified in the retinal vascular tissue of the AGE-RSA-injected group. The average number of retinal capillary pericytes per 10x100 microscope power field was 4.313+/-0.34 (mean +/- SD) in the AGE-RSA-injected group, compared with 5.798+/-0.481 in the control group ( P<0.01).

CONCLUSION

These experiments demonstrate that AGEs, independent of other metabolic factors, can induce vascular change resembling that of diabetic retinopathy.

摘要

背景

糖尿病视网膜病变已被证明与高血糖的程度和持续时间直接相关，晚期糖基化终产物（AGEs）也参与了这一病理过程。本文报道的实验目的是研究AGE沉积对导致糖尿病视网膜病变的视网膜血管损伤的影响。

方法

通过静脉注射外源性AGEs来处理野生型非糖尿病Sprague-Dawley大鼠。对每只动物的两张视网膜玻片之一进行免疫组织化学染色以标记视网膜血管AGE沉积，另一张进行苏木精-伊红（H&E）染色以计数毛细血管周细胞。将结果与未处理的野生型和糖尿病对照组以及用未修饰的大鼠血清白蛋白（RSA）处理的大鼠的结果进行比较。