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尿黏多糖的测定

Measurement of urinary mucopolysaccharides.

作者信息

du Plooy M, de Villiers L S

出版信息

S Afr Med J. 1975 Jun 21;49(26):1038-40.

PMID:125458
Abstract

The fact that the polyanionic long chains of mucopolysaccharides (MPS) can be precipitated with detergents such as cetylpiridiniumchloride (CPC) has been used in the development of a method for the quantitative estimation of total MPS in urine. Urine samples are all brought to an osmolarity of 300, after which an equal volume of 0,1% CPC is added to each sample. The turbidity is determined spectrophotometrically after 5 minutes. Chondroitin sulphate A was used as an internal standard. Normal values obtained for different age groups in the population of Pretoria are discussed.

摘要

粘多糖(MPS)的聚阴离子长链可被诸如十六烷基氯化吡啶(CPC)之类的去污剂沉淀,这一事实已被用于开发一种定量估算尿液中总MPS的方法。将所有尿液样本的渗透压调至300,之后向每个样本中加入等体积的0.1% CPC。5分钟后用分光光度法测定浊度。硫酸软骨素A用作内标。文中讨论了在比勒陀利亚人群中不同年龄组获得的正常值。

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