Newton D J, Scott J E, Ahmad S
Connect Tissue Res. 1979;7(1):47-55. doi: 10.3109/03008207909152352.
Urine from normal human adults (11 males, 4 females) was collected for 24 hours in four-hour samples, commencing at 08.00 hours. The urine volume, and concentrations of chondroitin sulfate, heparan sulfate, cetylpyridinium turbidity, and creatinine were measured on every sample. Concentrations and total output of glycosaminoglycans were significantly higher in male urine than in female urine. Chondroitin sulfate total output/four hours showed a significant negative correlation with creatinine concentration in males, but not in females. A testicular hyaluronidase is implicated. No such correlation was observed for heparan sulfate. Glycosaminoglycans are filtered into the urine. Plasma clearances are very low. Heparan sulfate is excreted with a circadian rhythm, as is glycosaminoglycan assayed by cetyl pyridinium turbidity. Peak excretions are at 06.00 and 10.00 hours respectively. Chondroitin sulfate excretion is not rhythmic in the male, perhaps because hyaluronidase activity in the urine complicates the assay. A rhythm may be present in the female.
收集11名男性和4名女性正常成年人的尿液，每4小时取样一次，共收集24小时，从上午8点开始。对每个样本测量尿量以及硫酸软骨素、硫酸乙酰肝素、十六烷基吡啶浊度和肌酐的浓度。男性尿液中糖胺聚糖的浓度和总排出量显著高于女性尿液。男性每4小时硫酸软骨素的总排出量与肌酐浓度呈显著负相关，而女性则无此相关性。这可能与睾丸透明质酸酶有关。硫酸乙酰肝素未观察到这种相关性。糖胺聚糖会滤入尿液。血浆清除率非常低。硫酸乙酰肝素的排泄具有昼夜节律，用十六烷基吡啶浊度测定的糖胺聚糖排泄也有昼夜节律。排泄高峰分别在上午6点和10点。男性硫酸软骨素的排泄没有节律，可能是因为尿液中的透明质酸酶活性使检测变得复杂。女性可能存在节律。
